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Macrophage Chemokine Ligand 2 (CXCL2) Competition ELISA Kit (MTS-1123-HM39)

Overview

Description
Creative Biolabs provides competition ELISA kit for quantitative measurement of Chemokine Ligand 2 (CXCL2) in different sample types by colorimetric.
Applications
ELISA
Qualified With
Quality Certificate
Detection Method
Colorimetric
Method Type
Competition ELISA
Analytical Method
Quantitative
Specificity
Chemokine Ligand 2 (CXCL2)

Specification

Size
96 tests
Sample Volume
100 μL
Assay Time
1.5 h
Plate
Pre-coated
Bioassay Target Name
Chemokine Ligand 2 (CXCL2)
Storage
4 °C
Storage Comment
Reference to the protocol
Expiry Date
6 months
Product Disclaimer
This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name
C-X-C motif chemokine ligand 2
Synonyms
GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
Background
This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation.
Sub Cat Reactivity Sensitivity Detection Range  
MTS-1123-HM322 Pig 100-2500 pg/mL Inquiry
MTS-1123-HM323 Cow User optimized Inquiry
MTS-1123-HM324 Goat User optimized Inquiry
MTS-1123-HM325 Human 25-500 pg/mL Inquiry
MTS-1123-HM326 Chicken User optimized Inquiry
MTS-1123-HM327 Monkey User optimized Inquiry
MTS-1123-HM328 Sheep 250-5000 pg/mL Inquiry
MTS-1123-HM329 Rat 250-5000 pg/mL Inquiry
FAQs Customer Reviews Related Products

What does the CXCL2 competition ELISA offer compared with sandwich ELISA?

The indexed description states this is a competition ELISA kit with colorimetric detection for quantitative measurement of CXCL2. Competitive designs can be useful when you prefer that assay principle or want an alternative to sandwich ELISA in your workflow. Keep in mind that competitive assays often have inverse signal behavior, so standards and controls are especially important for correct interpretation.

How can I prevent misinterpretation of competitive ELISA results in a large experiment?

In large runs, errors most often come from curve direction confusion, inconsistent timing, or choosing dilutions that land on insensitive curve regions. We recommend a pilot plate first: confirm the inverse relationship on your standard curve, then select a dilution that places most samples in the steep part of the curve. Include duplicates and a reference control on every plate. These steps dramatically reduce the chance of wrong conclusions in big datasets.

Can the competition kit support quantitative reporting suitable for publications?

Yes, it's described for quantitative measurement, but publication-quality reporting depends on execution: duplicates, complete standard curves, and documented quality checks (CV thresholds, control acceptance ranges). If you're comparing multiple matrices (e.g., plasma vs supernatant), validate dilution linearity and recovery for each matrix separately. For high-stakes endpoints, consider confirming a subset with an orthogonal approach to strengthen confidence and reviewer acceptance.

  • Competitive CXCL2 assay gave strong quantitative trends after one pilot optimization run
    We tried the competition kit because we wanted an alternative assay design for CXCL2. The first pilot plate helped us choose the right dilution range and confirm the curve direction. After that, the assay produced consistent quantitative patterns between treatment groups and correlated with other inflammatory readouts we measured. Including a reference control each plate was important for stability. Overall, it's reliable once you treat the first run as optimization.
  • Helpful option when you prefer competitive ELISA workflow over sandwich format
    Our lab already runs several competitive assays, so this CXCL2 kit fit our habits well. The colorimetric readout was easy and didn't require special equipment. We found that strict timing consistency mattered more than expected, but once standardized, plate-to-plate variation was low. It's a good kit for teams comfortable with competitive ELISA interpretation and looking for quantitative CXCL2 measurement.
  • Good quantitative CXCL2 detection in complex samples, provided controls are strong
    We tested this kit with complex matrices and found it could still deliver usable quantitative separation between groups, but only when controls were carefully planned. Duplicates and a consistent pooled reference control improved our confidence. The competitive format requires attention to the inverse curve, so we trained new team members using a pilot dataset before running the full study. With those steps, performance was stable and analysis was reproducible.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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