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Macrophage Interleukin 10 (IL10) ELISA Kit, qPCR (MTS-1123-HM72)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Interleukin 10 (IL10) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Interleukin 10 (IL10)

Specification

Size

96 tests

Bioassay Target Name

Interleukin 10 (IL10)

Storage

-20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 10

Synonyms

CSIF; TGIF; GVHDS; IL-10; IL10A

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM863	Human	User optimized		Inquiry
MTS-1123-HM864	Dog	0.01 ng/mL		Inquiry
MTS-1123-HM865	Rat	1 pg/mL		Inquiry
MTS-1123-HM866	Mouse	4.5 pg/mL		Inquiry
MTS-1123-HM867	Cat	0.02 ng/mL		Inquiry
MTS-1123-HM868	Chicken	1.6 pg/mL		Inquiry
MTS-1123-HM869	Horse	9 pg/mL		Inquiry
MTS-1123-HM870	Cow	User optimized		Inquiry

FAQs Customer Reviews Related Products

The page says "sandwich ELISA" but detection is "qPCR." What does that mean for my workflow?

This kit is described as a sandwich ELISA with qPCR detection and semi‑quantitative measurement. In practice, it pairs immunocapture specificity with nucleic-acid amplification-based readout for enhanced detection behavior in certain setups. Because it's labeled semi‑quantitative, we recommend using it for relative comparisons (treated vs. control) and consistent internal controls rather than absolute concentration claims unless validated in your hands.

When would semi‑quantitative IL‑10 measurement be preferable to standard colorimetric quantification?

Semi‑quantitative approaches are useful when your primary goal is comparative profiling across conditions or time points, especially if you expect low abundance and want a different detection modality than absorbance. This kit is positioned for semi‑quantitative IL‑10 measurement via qPCR. For pathway studies or screening where fold-change is more meaningful than exact pg/mL, this can be a practical choice.

Can you advise how to validate results against our existing IL‑10 ELISA data?

We suggest running a small bridging study: test the same sample set with your existing quantitative ELISA and this qPCR-detection, semi‑quantitative sandwich format. Compare rank ordering and fold-changes across conditions, and confirm linearity across a dilution series. If trends match while absolute values differ, that's expected with modality changes; you can then standardize reporting around relative changes for consistency.

Helpful semi‑quantitative kit for IL‑10 trend tracking across experiments
We used this kit when our main need was to compare IL‑10 levels between macrophage polarization conditions rather than publish absolute concentrations. The qPCR-based detection gave clear directional differences between groups. After we established consistent controls and a dilution scheme, the results were stable across runs. It's best treated as a relative measurement tool, and it served that purpose well.
Good alternative readout when colorimetric ELISA sensitivity was limiting
In some of our samples, colorimetric ELISA signals were close to background, so we explored this qPCR-detection option. The kit is described as a semi‑quantitative sandwich ELISA, and it produced clearer separation across conditions once optimized. It required careful workflow discipline, but it was a useful complement to our standard assays for low-signal scenarios.
Strong for relative IL‑10 comparisons with consistent internal controls
We adopted this kit for screening many experimental conditions quickly, focusing on relative IL‑10 changes. With a stable control sample included on every plate, we saw reproducible patterns over time. The qPCR readout fit our lab's existing instrumentation and reduced reliance on absorbance-only interpretation. For projects centered on comparisons rather than absolute quantitation, it worked smoothly.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.