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Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) ELISA Kit, Colorimetric (MTS-1123-HM54)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Specificity

Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

colony stimulating factor 2

Synonyms

CSF; GMCSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM381	Mouse		1.37 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM382	Human		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM383	Rat		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM384	Cow		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM385	Rabbit		7.813 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM386	Dog		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM387	Rat		15.6-1000 pg/mL	Inquiry
MTS-1123-HM388	Mouse		15.6-1000 pg/mL	Inquiry
MTS-1123-HM389	Pig		72-18.000 pg/mL	Inquiry
MTS-1123-HM390	Mouse		0.03-2.0 ng/mL	Inquiry
MTS-1123-HM391	Rat		0.02-1.0 ng/mL	Inquiry
MTS-1123-HM392	Guinea Pig		25-500 pg/mL	Inquiry
MTS-1123-HM393	Monkey		50-1000 pg/mL	Inquiry
MTS-1123-HM394	Rabbit		25-500 pg/mL	Inquiry
MTS-1123-HM395	Rhesus Monkey		User optimized	Inquiry
MTS-1123-HM396	Cat		User optimized	Inquiry
MTS-1123-HM397	Pig		15.6-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is this kit suitable for cytokine release experiments with many replicates and timepoints?

Yes. Colorimetric sandwich ELISA is commonly used for high-throughput cytokine profiling because the workflow scales well and the readout is compatible with standard plate readers. For large studies, we recommend building an SOP with fixed timing, using multichannel pipettes, and including a consistent QC sample on each plate. These steps make time-course data more comparable and reduce operator-driven variability.

Can I compare CSF2 values between serum and cell supernatants directly?

Direct comparison across matrices can be misleading due to matrix effects. We recommend validating each matrix separately with spike-and-recovery and dilution linearity checks. If you must compare across matrices, use matrix-matched standards or report results as within-matrix comparisons. We can suggest a validation plan so your reported differences reflect biology rather than assay interference.

How can I reduce non-specific background when samples contain high protein content?

High protein content can increase background through non-specific binding. Typical mitigations include sample dilution, improved washing (volume and cycles), proper blocking and plate sealing, and ensuring reagents are fully equilibrated. Using clarified samples (centrifuged to remove debris) often helps as well. If background persists, we can advise workflow adjustments and control placement to identify whether the source is matrix, wash efficiency, or timing.

Great for GM-CSF monitoring in stimulated immune-cell supernatants
We used the kit for CSF2 measurement after TLR stimulation and saw clear, consistent differences between conditions. The colorimetric readout was easy on our shared reader, and the assay scaled well to multiple timepoints. Including a pooled supernatant QC on each plate improved confidence in longitudinal comparisons. It's now part of our routine panel for cytokine release profiling.
Clean curves and manageable background after adopting recommended dilution strategy
Early runs in serum-rich media had higher background, but spike recovery and dilution linearity testing helped us choose a stable dilution factor. After that, standard curves and replicates looked much better. The kit gave us reliable trends for decision-making, and the protocol was easy to train new staff on. Overall, good value for routine cytokine quantification.
Consistent performance with strong cross-plate reproducibility using internal controls
Our study required dozens of plates across several weeks. We controlled timing tightly and used an internal QC sample to bridge plates. That approach produced stable signals and reduced the need for reruns. The kit's workflow is familiar, and with good washing technique it delivers reproducible CSF2 data suitable for comparative analyses and reporting.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.