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Macrophage Serine/Threonine Protein Kinase (STK) ELISA Kit, Colorimetric (MTS-1123-HM4)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Serine/Threonine Protein Kinase (STK) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sensitivity

9.375 ng/mL

Sample Type

Plasma, Serum, Tissue Homogenate

Specificity

Serine/Threonine Protein Kinase (STK)

Specification

Size

96 tests

Detection Range

15.625 ng/mL-1000 ng/mL

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Serine/Threonine Protein Kinase (STK)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

serine/threonine protein kinase-9

Background

A serine/threonine protein kinase (EC 2.7.11.-) is a kinase enzyme, in particular a protein kinase, that phosphorylates the OH group of the amino-acid residues serine or threonine, which have similar side chains. At least 350 of the 500+ human protein kinases are serine/threonine kinases (STK).

FAQs Customer Reviews Related Products

Kinases can exist in different phosphorylation states. Does this kit measure total STK protein or an activated/phosphorylated form?

This product is presented as a colorimetric sandwich ELISA for the target "Serine/Threonine Protein Kinase (STK)." Unless the datasheet explicitly specifies a phospho-epitope, you should assume it measures the target protein level rather than a specific phosphorylation state. If your goal is pathway activation, pair this assay with a phospho-specific method (e.g., phospho-Western or phospho-ELISA) while using this kit to track total protein changes.

Can I use nuclear/cytosolic fractionation samples, and what should I watch out for?

Fractionated samples can work well, provided the buffer composition is compatible and fractions are clear of particulates. The main risks are high salt, strong detergents, or carryover of inhibitors that increase background or reduce binding. We suggest dialyzing or diluting fractions into a compatible buffer, running a dilution series to confirm parallelism, and keeping total protein input comparable across fractions so your readout reflects true distribution changes rather than loading differences.

I need high throughput for dozens of conditions. What's the most common reason for edge effects and how do I prevent them?

Edge effects usually come from uneven temperature, evaporation, or inconsistent plate washing. To reduce them, equilibrate reagents to the same temperature, seal plates during incubation, avoid placing plates near drafts, and use consistent wash volume and aspiration technique. If you have enough samples, consider leaving outer wells for blanks/controls. These simple practices usually improve uniformity and make high-throughput comparisons much more reliable.

Good total-protein readout for STK with consistent plate handling
We used the kit to track STK protein changes after drug treatment in macrophage-like cells. The assay behaved like a typical sandwich ELISA, and results were repeatable once we standardized wash steps and incubation timing. It's best viewed as a total-protein measurement rather than an activation assay; for phosphorylation, we used a separate phospho method. As a screening tool, it helped us prioritize conditions quickly.
Scales well for multi-condition studies after minor buffer tweaks
Our initial lysate buffer produced slightly higher background, but reducing detergent and adding a clarification spin solved it. The colorimetric output was stable and easy to read on our standard plate reader. I like that it doesn't require specialized detection hardware, so we could run multiple plates in one day. Overall performance was solid for comparing trends across many experimental groups.
Straightforward kit that benefits from including an internal control sample
We ran several plates over time and found that including a pooled lysate control on each plate improved comparability dramatically. The kit produced consistent curves and the protocol was familiar to anyone doing ELISAs. When we kept incubation conditions constant and avoided plate edge evaporation, variability was minimal. This made it a useful tool for routine monitoring of STK levels during macrophage activation experiments.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.