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Macrophage IL-1 beta ELISA Kit, qPCR (MTS-1123-HM66)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of IL-1 beta (IL1B) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

IL-1 beta (IL1B)

Specification

Size

96 tests

Bioassay Target Name

IL-1 beta (IL1B)

Storage

-20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 1 beta

Synonyms

IL-1; IL1F2; IL1beta; IL1-BETA

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM840	Human	User optimized		Inquiry
MTS-1123-HM841	Rat	8 pg/mL		Inquiry
MTS-1123-HM842	Rabbit	4.5 pg/mL		Inquiry
MTS-1123-HM843	Pig	0.6 pg/mL		Inquiry
MTS-1123-HM844	Mouse	0.5 pg/mL		Inquiry
MTS-1123-HM845	Dog	1 pg/mL		Inquiry
MTS-1123-HM846	Cat	1.6 pg/mL		Inquiry
MTS-1123-HM847	Sheep	User optimized		Inquiry
MTS-1123-HM848	Dolphin	User optimized		Inquiry
MTS-1123-HM849	Horse	User optimized		Inquiry
MTS-1123-HM850	Cow	User optimized		Inquiry
MTS-1123-HM851	Rhesus Monkey	User optimized		Inquiry

FAQs Customer Reviews Related Products

I'm running macrophage polarization studies-how should I integrate this IL-1β qPCR kit with my existing cytokine panel?

A practical approach is to use IL-1β as a high-impact inflammatory marker alongside TNF-α, IL-6, and chemokines. In many workflows, the "qPCR" positioning supports interpretation alongside transcriptional signatures. We recommend aligning sample collection time points between secretion measurements and RNA extraction. That way, you can connect IL-1β output with upstream pathway activation, rather than treating each dataset in isolation.

Is this kit better for relative comparisons or absolute cytokine quantitation?

Most teams get the best value from using it for within-study comparisons: control vs stimulated, inhibitor titrations, or time-course ranking. Absolute quantitation across unrelated experiments is always more sensitive to operator differences and matrix effects. If absolute numbers are critical, we suggest strict standardization: same plate reader settings, consistent incubation timing, and an internal reference sample included on every plate to verify run-to-run stability.

What troubleshooting steps help if IL-1β values don't match our expected biology?

First confirm sample timing: IL-1β secretion can be highly time-dependent and may require priming + activation. Second, verify sample integrity by avoiding repeated freeze-thaw and clarifying debris. Third, check dilution linearity-if a sample is too concentrated, you can see signal compression or unexpected trends. Finally, include biological controls (known activator and known inhibitor) to confirm the assay is reflecting biology rather than handling artifacts.

Supported a coherent story between IL-1β secretion patterns and gene signatures
We used this kit in a project where we wanted cytokine output to match our transcriptional signatures. It helped us verify that IL-1β changes tracked with inflammasome-related gene patterns in the same conditions. The best practice for us was synchronized sampling and consistent handling across runs. Once we did that, the data were stable and helped strengthen the mechanistic narrative in our report.
Useful for ranking conditions in a time-course rather than chasing absolute numbers
The kit worked best when we focused on time-course trends and relative differences across stimuli. IL-1β can be sensitive to timing, so once we standardized collection points, we saw consistent condition ranking. It saved time in decision-making because we could confidently choose which treatments to follow up with deeper assays. Overall, it's a practical tool for screening macrophage inflammatory outputs.
Reduced rework after we applied a simple sample clarification and dilution rule
Early on, we had variability that turned out to be sample handling related. After implementing a standard spin step and using a consistent dilution factor across similar sample sets, the variability dropped. The assay then produced repeatable IL-1β results aligned with our controls. We appreciated that it fit smoothly into our broader workflow without requiring specialized instrumentation changes.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.