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Macrophage Chemokine Ligand 2 (CCL2) ELISA Kit, qPCR (MTS-1123-HM84)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 2 (CCL2) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 2 (CCL2)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 2 (CCL2)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 2

Synonyms

HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

Background

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM887	Human, Rhesus Monkey	0.8 pg/mL		Inquiry
MTS-1123-HM888	Mouse, Human, Dog	0.9 pg/mL		Inquiry
MTS-1123-HM889	Cow	0.131 ng/mL		Inquiry
MTS-1123-HM890	Mouse	0.3 pg/mL		Inquiry
MTS-1123-HM891	Rat	1.5 pg/mL		Inquiry
MTS-1123-HM892	Horse	0.82 pg/mL		Inquiry
MTS-1123-HM893	Human	0.032 pg/mL		Inquiry
MTS-1123-HM894	Pig	2.8 pg/mL		Inquiry

FAQs Customer Reviews Related Products

What are the core specs I should plan around-plate type, sample volume, and storage?

This kit is listed as 96 tests, pre-coated plate, with 25 µL sample volume per well and storage options at 4°C, -20°C, and -80°C, depending on reagent type per protocol. It's described as a sandwich ELISA with qPCR detection and a semi-quantitative analytical method. For planning, pilot your dilution factor, then keep sample handling consistent to preserve comparability across runs.

What sample types are supported for typical macrophage projects?

The listing specifies cell culture supernatant, plasma, and serum as sample types. In macrophage experiments, supernatants are most common; we recommend clarifying by centrifugation and using consistent collection timing (e.g., 24h post-stimulation). For plasma/serum, avoid hemolysis, freeze in aliquots, and run dilution checks since matrix effects can differ from culture media.

Is "semi-quantitative" a limitation for publications or screening only?

Semi-quantitative does not automatically mean "non-publishable." It typically means the method is optimized for robust relative comparisons across conditions rather than claiming absolute concentration with the same precision as fully quantitative colorimetric methods. Many labs publish semi-quantitative data when they include appropriate controls, replicate structure, and clear statistical comparisons. If you need absolute concentrations, consider running a bridging set with a fully quantitative colorimetric kit as confirmation.

Excellent for relative CCL2 trends with low sample volume needs
We had limited macrophage supernatant volumes, so the 25 µL requirement was a big advantage. The qPCR-based detection format fit our lab's workflow, and the results captured clear differences between treatments. After setting a consistent dilution, the assay was reproducible across days. We treated it as a semi-quantitative tool for ranking conditions, and it delivered exactly what we needed.
Pre-coated plate saved setup time and improved consistency across users
Multiple people in our lab ran this kit, and the pre-coated plate format helped standardize results. We added an internal control sample on each plate and saw stable readouts. It's best used for comparing groups rather than claiming absolute concentrations, but for screening and mechanistic studies, it worked well and reduced hands-on variability.
Strong fit for macrophage studies when you want semi-quantitative comparisons
Our project focused on whether interventions reduced CCL2 output, not on precise pg/mL values. This kit supported that goal well. The storage flexibility was helpful for managing reagents, and the sample types matched our needs. Once we kept timing and washing consistent, the assay produced clean separation between groups and minimized re-runs.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.