Macrophage Chemokine Ligand 16 (CCL16) ELISA Kit, Colorimetric (MTS-1123-HM96)

The colorimetric format is designed for standard lab instrumentation and typical macrophage culture workflows. In most cases, you can measure CCL16 in supernatants using a conventional absorbance plate reader, with performance depending on proper dilution and consistent washing. If your media contains serum or other complex components, we recommend a pilot dilution series and recovery checks to ensure accurate quantification. We can help you plan controls for your exact culture conditions.

To reduce edge effects, avoid using outer wells for critical samples if your lab environment has temperature gradients, or fill edge wells with buffer when appropriate. Keep incubation times consistent across the plate and use a multichannel pipette for uniform addition. Running duplicates and including a shared reference sample across plates also helps confirm that shifts reflect biology rather than procedural drift. We can suggest a plate map layout optimized for your sample count.

A simple and effective approach is spike-and-recovery: add a known amount of CCL16 standard into a representative sample matrix and verify that the measured value matches expectations within an acceptable range. Also run a dilution linearity check to confirm that dilution reduces signal proportionally. These two checks quickly reveal whether the matrix is interfering. If you share your media composition and sample prep steps, we can recommend an efficient validation plan.