Macrophage Expressed Gene 1 (MPEG1) ELISA Kit, Colorimetric (MTS-1123-HM9)

Many teams use macrophage-associated proteins as practical biomarkers, but timing depends on your model and stimulus. We suggest designing a small time-course first (e.g., early, mid, late points) and running the ELISA on matched sample types (lysate or supernatant) to identify when MPEG1 changes are most dynamic. Use consistent normalization (total protein for lysates, volume/time for supernatants) so changes reflect biology rather than sampling variation.

For lysates, normalize input by total protein and keep loading consistent across wells. If donor variability is large, run a dilution series for a subset to confirm parallelism and choose a dilution that keeps most samples in the curve's mid-range. Including a pooled donor control on each plate can help anchor comparisons. For supernatants, normalize by cell number or culture duration to avoid confounding secretion differences with culture density.

Standard singleplex sandwich ELISAs are typically run separately to avoid cross-reactivity and signal overlap, especially in colorimetric formats. If sample volume is limited, you can still plan efficiently: aliquot once, minimize freeze-thaw, and coordinate runs so all ELISAs use the same sample preparation. If true multiplexing is required, consider a dedicated multiplex platform; otherwise, separate plates usually provide the cleanest and most interpretable results.