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Macrophage Interleukin 12 (IL12) ELISA Kit, Colorimetric (MTS-1123-HM76)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Interleukin 12 (IL12) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Interleukin 12 (IL12)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3.5 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 12 (IL12)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 12

Synonyms

IL-12

Background

Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12. IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM580	Rat		15.63 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM581	Rabbit		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM582	Chicken		0.78 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM583	Human		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM584	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM585	Human		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM586	Mouse		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM587	Dog		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM588	Guinea Pig		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM589	Monkey		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM590	Sheep		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM591	Hamster		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM592	Chicken		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM593	Pig		0.45 ng/mL-30 ng/mL	Inquiry
MTS-1123-HM594	Rhesus Monkey		40-10000 pg/mL	Inquiry
MTS-1123-HM595	Human		31.25-2000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is this IL‑12 kit meant for absolute quantification, and what detection approach does it use?

The product is described as a sandwich ELISA kit for quantitative measurement of IL‑12 using a colorimetric method. In practical terms, you'll generate a standard curve and calculate concentrations from absorbance values. For absolute quantification, ensure your samples fall within the effective curve range, run replicates, and keep incubation/washing consistent to avoid drift in the curve or inflated background.

We work with macrophage stimulation models. Does this kit align with common macrophage cytokine profiling needs?

Yes-IL‑12 is a key cytokine in macrophage and antigen-presenting cell responses, and a quantitative colorimetric sandwich ELISA is commonly used for profiling stimulated vs. baseline conditions. We recommend including time-course sampling and running controls for stimulation strength. If you plan to compare across multiple batches, keep the same kit lot and repeat an internal reference sample on every plate.

How do I decide between IL‑12 colorimetric quantification and a qPCR-detection ELISA option?

Choose colorimetric quantitative ELISA when you want straightforward absorbance-based absolute concentrations and a well-established workflow. Consider qPCR-detection ELISA when you prioritize relative comparisons or need an alternative readout modality. Your decision should reflect instrumentation availability, desired data type (absolute vs. relative), and how you will validate performance in your matrices.

Solid quantitative IL‑12 kit for macrophage stimulation profiling experiments
We used this kit to quantify IL‑12 after macrophage stimulation in vitro. The sandwich colorimetric format is familiar and easy to integrate into routine workflows, and the quantitative intent was clear: standards plus unknowns in replicate. After we standardized incubation timing, the results were stable and supported our comparison across stimuli. It worked well as a core assay for cytokine profiling.
Consistent readout and easy integration into an ELISA-heavy pipeline
Our lab runs many ELISAs weekly, and this IL‑12 kit fit seamlessly into our pipeline. The quantitative colorimetric setup produced interpretable data with minimal troubleshooting. We appreciated that it behaved like a standard sandwich ELISA, making it easy to train staff and maintain consistent procedures. With careful sample dilution planning, we obtained reliable IL‑12 concentration estimates across multiple experiments.
Good performance for comparative and absolute IL‑12 measurements across batches
We used the kit for both absolute measurements (pg/mL style reporting) and to compare relative changes across different stimulation time points. Because it's positioned as quantitative, we emphasized consistent standard preparation and included an internal reference control on every plate. This improved comparability across runs and helped us detect subtle IL‑12 shifts without excessive variance.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.