Macrophage Chemokine Ligand 19 (CCL19) ELISA Kit, Colorimetric (MTS-1123-HM14)

Because this kit supports multiple matrices (including serum and tissue homogenate), the best way to control matrix effects is to standardize sample handling and confirm performance using in-run controls. For tissues, keep homogenization conditions consistent (buffer, protease inhibitors if applicable, and centrifugation to remove debris), then consider testing at least two dilutions to ensure your readings fall within the linear portion of the curve. For serum/plasma, avoid repeated freeze-thaw cycles and clarify samples by centrifugation before loading. If you see nonlinearity upon dilution, that often indicates matrix interference-dilution and proper sample diluent selection typically resolves it.

The assay is designed for quantitative measurement with a defined working range (the datasheet lists sensitivity/detection range for supported species options). If a sample's signal is above the top standard, the most reliable correction is dilution-dilute the sample in the recommended diluent and re-run so the result falls inside the validated curve. We also recommend running samples in duplicate and including an internal control sample across plates to monitor run-to-run consistency. If you anticipate very high CCL19 (for example, strongly stimulated immune cultures), planning a pilot run with serial dilutions can save time and conserve standards.

For routine profiling where you want a familiar workflow and straightforward OD-based quantification, the colorimetric sandwich ELISA is typically the best first choice. It uses a standard plate-reader readout, accommodates a wide set of sample types (including lysates and tissue homogenates), and fits well into established immunology pipelines. A qPCR-readout option can be attractive when sample volume is extremely limited or when you need to push lower abundance detection, but it introduces additional instrument requirements and workflow steps. If your primary goal is robust day-to-day comparability, colorimetric is usually the most practical baseline.