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Macrophage TNF alpha ELISA Kit, qPCR (MTS-1123-HM63)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Tumor Necrosis Factor alpha (TNF alpha) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Tumor Necrosis Factor alpha (TNF alpha)

Specification

Size

96 tests

Bioassay Target Name

Tumor Necrosis Factor alpha (TNF alpha)

Storage

-20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Synonyms

TNF; TNF-α

Background

Tumor necrosis factor (TNF, cachexin, or cachectin; formerly known as tumor necrosis factor alpha or TNF-α) is an adipokine and a cytokine. TNF is a member of the TNF superfamily, which consists of various transmembrane proteins with a homologous TNF domain.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM832	Human	User optimized		Inquiry
MTS-1123-HM833	Dog	0.2 pg/mL		Inquiry
MTS-1123-HM834	Mouse	10 pg/mL		Inquiry
MTS-1123-HM835	Pig	2 pg/mL		Inquiry
MTS-1123-HM836	Rhesus Monkey	4 pg/mL		Inquiry
MTS-1123-HM837	Rat	2.5 pg/mL		Inquiry
MTS-1123-HM838	Cow	0.01 ng/mL		Inquiry
MTS-1123-HM839	Dolphin	User optimized		Inquiry

FAQs Customer Reviews Related Products

The page says "qPCR" and "sandwich ELISA"-what should I expect as the final readout?

This kit is positioned for workflows where TNF-α profiling is integrated with qPCR-style analysis pipelines. The method type is listed as sandwich ELISA, with qPCR as the detection method and semi-quantitative analytical method. Practically, customers typically use it to compare relative changes across conditions (e.g., stimulated vs control) rather than relying solely on absolute concentrations across unrelated studies.

What sample types are recommended for macrophage inflammation experiments?

The listed sample types are cell culture supernatant, plasma, and serum. For macrophage activation experiments, supernatant is usually the cleanest and most direct measure of secreted TNF-α. If using plasma/serum, we advise consistent anticoagulants, matched collection timing, and a small pilot dilution test to ensure the matrix does not compress your signal range.

How should I store the kit if I plan to run multiple plates over a few months?

The storage condition is -20°C, and the expiry is listed as 6 months. To preserve performance, aliquot any reconstituted reagents if applicable, avoid repeated freeze-thaw, and bring components to working temperature consistently before use. Keeping a simple run log (operator, incubation times, reader settings) also helps maintain comparability across plates over time.

Strong relative TNF-α trends that supported our qPCR interpretation workflow
We needed a TNF-α assay that helps us interpret secretion changes alongside our gene-expression signatures. This kit delivered consistent differences between groups, which was our main goal. The sample types aligned well with our study (supernatant plus a small serum subset). It worked best when we treated it as a semi-quantitative tool-excellent for ranking conditions and confirming directionality.
Convenient for multi-condition macrophage screens with clear handling instructions
We screened multiple stimuli and inhibitors and wanted a kit that wouldn't slow us down. The workflow was manageable and the reagents behaved consistently when we followed timing carefully. Storage at -20°C and the 96-test format made planning easy. Once we standardized our dilution approach, results were reproducible across operators, which is critical in a shared lab environment.
Best value when you prioritize consistent comparisons over absolute quantitation
We initially expected "absolute numbers," but the kit really shines for consistent comparisons within a project. After aligning expectations, we appreciated how stable the rank-ordering of samples was across runs. It helped validate that our macrophage activation signatures had a corresponding secretion phenotype. If your goal is biological conclusions rather than cross-study absolute benchmarking, it's a good match.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.