Macrophage Stimulating 1 Receptor ELISA Kit, Colorimetric (MTS-1123-HM44)

This kit is a sandwich ELISA designed for quantitative measurement of Macrophage Stimulating 1 Receptor with a colorimetric readout. In ELISA, what you detect depends on antibody epitope recognition and the form present in your sample (lysate vs supernatant). For membrane-associated receptors, lysates often provide clearer detection, while supernatants may reflect shedding. We recommend running both matrices initially and validating interpretation with controls.

When comparing primary cells and cell lines, the best practice is to standardize sample input (total protein normalization for lysates) and use consistent dilution schemes so readings sit within the reliable region of the standard curve. Because this kit is quantitative, it supports cross-sample comparisons if assay conditions are controlled. Including a reference sample (e.g., pooled lysate) across plates also helps track variability and improves comparability over time.

While the kit is supplied for research use, we can still support robust validation planning: accuracy by spike recovery, precision by repeated runs, and matrix evaluation through dilution linearity. We also help define acceptance criteria aligned with your internal QA framework. If you share your matrix, expected concentration range, and required precision thresholds, we can propose a validation checklist and documentation strategy suitable for stringent internal standards.