This competition ELISA kit is particularly well-suited for detecting low-abundance CCL13 in macrophage-driven inflammatory models. The competitive format minimizes steric hindrance and improves detection reliability when antigen concentrations are limited. It supports serum, plasma, and cell culture supernatants. We recommend performing preliminary dilution optimization to ensure sample readings fall within the linear range of the standard curve for accurate and reproducible quantification.
The competitive format is advantageous for small chemokines like CCL13 where epitope accessibility may be limited. It reduces interference from sample matrix components and allows accurate quantification even in complex biological fluids. Although signal intensity is inversely proportional to antigen concentration, the kit includes detailed data analysis guidance to ensure reliable curve fitting and precise results.
This kit undergoes strict quality control validation. Intra-assay and inter-assay variability are maintained at low levels to ensure consistency. Each batch includes calibrated standards and validation documentation. When the protocol is followed carefully, users can expect stable standard curves and highly reproducible quantitative results across independent experiments.
For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.