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Macrophage Interleukin 10 (IL10) Competition ELISA Kit (MTS-1123-HM71)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Interleukin 10 (IL10) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Serum, Tissue Homogenate

Specificity

Interleukin 10 (IL10)

Specification

Size

96 tests

Sample Volume

50 μL

Assay Time

1 - 4.5 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 10 (IL10)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 10

Synonyms

CSIF; TGIF; GVHDS; IL-10; IL10A

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM543	Cow		5-1000 pg/mL	Inquiry
MTS-1123-HM544	Chicken		1-200 pg/mL	Inquiry
MTS-1123-HM545	Sheep		5-80 pg/mL	Inquiry
MTS-1123-HM546	Rabbit		50-1000 pg/mL	Inquiry
MTS-1123-HM547	Goat		50-1000 pg/mL	Inquiry
MTS-1123-HM548	Chicken		50-1000 pg/mL	Inquiry
MTS-1123-HM549	Mouse		50-1000 pg/mL	Inquiry
MTS-1123-HM550	Rat		50-1000 pg/mL	Inquiry
MTS-1123-HM551	Guinea Pig		50-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

When should I choose a competition ELISA instead of a sandwich ELISA for IL‑10?

A competition ELISA can be preferred when target size/epitope availability or sample conditions make two-antibody "sandwiching" less ideal, or when you want a robust approach that tolerates certain matrix effects. This kit is described as a colorimetric, quantitative competition ELISA for IL‑10. If you anticipate epitope masking or need a format compatible with specific assay constraints, competition can be a strong option.

What does "quantitative measurement by colorimetric" mean in practice-do I still build a standard curve?

Yes. Even in a competition format, quantitative interpretation relies on a well-prepared standard curve and consistent incubation/handling. The kit is designed for quantitative measurement with a colorimetric readout. In practice, you'll run standards and unknowns together, ensure proper mixing and timing, and use replicate wells to reduce variance-especially important in competition assays where signal decreases with higher analyte.

Can you help with troubleshooting if my curve looks "flat" or the dynamic range is compressed?

Absolutely. With competition ELISA, common causes of flattened curves include incorrect standard dilution series, insufficient mixing, incubation temperature deviations, or plate washing issues. Because the method is colorimetric and quantitative, small procedural inconsistencies can noticeably impact curve shape. We recommend verifying pipette calibration, preparing fresh standards, increasing wash stringency, and confirming that sample concentrations fall within the expected working range.

Competition format handled challenging samples better than our sandwich workflow
We switched to a competition IL‑10 ELISA because our sample matrix sometimes interfered with sandwich binding. This kit's quantitative colorimetric format worked well after we optimized dilutions. Once we got the standard curve stable, results aligned with our biological expectations in macrophage stimulation experiments. It required careful technique, but the output was dependable when we followed timing and washing precisely.
Solid quantification after initial learning curve for competition assays
If you're used to sandwich ELISA, competition assays can feel different because the signal goes the opposite direction with analyte concentration. After a couple of runs, this kit produced consistent quantitative results using a colorimetric readout. We liked the reproducibility once we standardized incubation time and mixing. For labs that need an alternative format, it's a good option.
Good kit for IL‑10 quantitation when epitope accessibility is a concern
We had concerns that certain treatments might alter IL‑10 binding availability in our assay conditions. The competition ELISA approach helped us obtain usable quantitative measurements without redesigning the whole workflow. The colorimetric detection was simple to implement. With proper dilution planning and replicates, we got clear group differences and improved confidence in our conclusions.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.