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Macrophage Chemokine Ligand 5 (CCL5) Competition ELISA Kit (MTS-1123-HM86)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Chemokine Ligand 5 (CCL5) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 5 (CCL5)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 5 (CCL5)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 5

Synonyms

SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta

Background

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM657	Cow		User optimized	Inquiry
MTS-1123-HM658	Monkey		50-1000 pg/mL	Inquiry
MTS-1123-HM659	Chicken		User optimized	Inquiry
MTS-1123-HM660	Sheep		User optimized	Inquiry
MTS-1123-HM661	Pig		User optimized	Inquiry
MTS-1123-HM662	Human		50-1000 pg/mL	Inquiry
MTS-1123-HM663	Goat		User optimized	Inquiry

FAQs Customer Reviews Related Products

What are the key specs-sample types, assay time, and storage-so I can plan my run day?

The kit is listed as competition ELISA, colorimetric, quantitative, with sample types including cell culture supernatant, plasma, serum, and tissue homogenate. It's 96 tests, uses 100 μL sample volume per well, and has an assay time of about 1.5 hours. Storage is listed at 4°C with 6 months expiry, so it's well-suited for routine scheduling.

Can I run tissue homogenates and serum together without compromising data quality?

You can run them on the same plate, but you should validate them separately because their matrix effects differ. We recommend separate dilution series and spike-and-recovery checks for tissue homogenate versus serum. If you must compare across matrices, interpret cautiously-matrix differences can shift apparent concentrations. For best results, keep each sample type within its validated dilution and use matrix-matched blanks.

How do I choose between this competition kit and the CCL5 sandwich options?

Choose based on your matrix complexity and study goals. Sandwich assays often provide strong specificity for many soluble targets, while competition assays can be helpful in certain sample contexts. If you need quantitative measurement across supernatant/serum/tissue homogenate and want a relatively fast protocol, this competition kit is a strong option. If your priority is maximum classical sandwich specificity in cleaner matrices, a sandwich format may be preferable.

Fast assay and robust results across macrophage supernatants and serum
We used this competition ELISA to quantify CCL5 in macrophage supernatants and a smaller serum set. The assay length made it easy to schedule, and the data were consistent once we optimized dilutions for each matrix. The colorimetric readout worked well with our plate reader, and we got clear differences between stimulation conditions. It's a practical kit for labs running mixed sample types.
Competition format handled our tissue homogenate variability better than expected
Tissue homogenates are always messy, and we usually fight matrix effects. After clarifying samples and running recovery checks, this kit produced stable curves and reasonable reproducibility. The differences between groups matched our histology trends, which increased confidence. It still requires disciplined sample prep, but it performed well in a challenging context and saved us time compared with re-running failed plates.
Good quantitative tool when you standardize controls and avoid freeze-thaws
We included a consistent internal control and avoided repeated freeze-thaw cycles of samples. With those precautions, the assay produced reliable quantitative outputs across multiple runs. The storage at 4°C was convenient. Overall, it became one of our go-to CCL5 quantitation methods for macrophage projects.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.