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Macrophage Interleukin 23 (IL23) ELISA Kit, Colorimetric (MTS-1123-HM79)

Overview

Description
Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Interleukin 23 (IL23) in different sample types by colorimetric.
Applications
ELISA
Qualified With
Quality Certificate
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum
Specificity
Interleukin 23 (IL23)

Specification

Size
96 tests
Sample Volume
100 μL
Assay Time
3.5 h
Plate
Pre-coated
Bioassay Target Name
Interleukin 23 (IL23)
Storage
4 °C, -20 °C
Storage Comment
Reference to the protocol
Expiry Date
6 months
Product Disclaimer
This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name
interleukin 23
Synonyms
IL-23
Background
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of an IL-12B (IL-12p40) subunit (which is shared with IL-12) and an IL-23A (IL-23p19) subunit. IL-23 is part of the IL-12 family of cytokines. The functional receptor for IL-23 (the IL-23 receptor) consists of a heterodimer between IL-12Rβ1 and IL-23R.
Sub Cat Reactivity Sensitivity Detection Range  
MTS-1123-HM604 Human 39.06 pg/mL-2500 pg/mL Inquiry
MTS-1123-HM605 Mouse 15.625 pg/mL-1000 pg/mL Inquiry
MTS-1123-HM606 Rat 15.625 pg/mL-1000 pg/mL Inquiry
MTS-1123-HM607 Pig 78.125 pg/mL-5000 pg/mL Inquiry
MTS-1123-HM608 Sheep 15.625 pg/mL-1000 pg/mL Inquiry
MTS-1123-HM609 Human 63-8000 pg/mL Inquiry
MTS-1123-HM610 Mouse 40-2500 pg/mL Inquiry
MTS-1123-HM611 Mouse 0.02-1.0 ng/mL Inquiry
MTS-1123-HM612 Dog 100-2500 pg/mL Inquiry
MTS-1123-HM613 Rabbit 100-2500 pg/mL Inquiry
MTS-1123-HM614 Guinea Pig 100-2500 pg/mL Inquiry
FAQs Customer Reviews Related Products

Does this kit work for both serum and cell culture supernatant IL‑23, and what is the assay format?

Yes. This kit is a sandwich ELISA, colorimetric, and quantitative, and lists cell culture supernatant, plasma, and serum as supported sample types. Sandwich format generally provides strong specificity via two antibodies. For best accuracy, clarify samples and consider dilution checks-especially for inflammatory serum where matrix effects can elevate background or compress the curve if samples are too concentrated.

What are the runtime, volume, and plate type? We need to plan staffing and instruments.

The kit uses a pre-coated plate, requires 100 µL sample volume, and the assay time is about 3.5 hours. This fits a typical half-day schedule. To reduce variability, use multichannel pipetting for uniform timing, wash consistently, and read absorbance promptly after substrate development. Including a full standard curve per plate improves confidence when comparing across multiple experiment days.

What are storage and expiry expectations for labs running IL‑23 intermittently?

Storage conditions are listed as 4°C and -20°C, with a 6‑month expiry. For intermittent usage, protect reagents from contamination and light where applicable, and avoid repeated freeze-thaw of standards. If you anticipate sporadic runs, plan to keep an internal control sample across plates to track assay drift and maintain comparability over time.

  • Strong quantitative IL‑23 performance with a clean, consistent standard curve
    We ran IL‑23 in serum and macrophage culture supernatant, and the kit's stated sample compatibility matched our needs. The 3.5‑hour workflow was manageable, and the pre-coated plate reduced hands-on time. Standard curves were consistent across duplicate plates, and we saw clear differences between stimulated and control groups. It's a dependable option for routine quantitative IL‑23 measurement.
  • Convenient pre-coated format and stable results across multiple operators
    Two different staff members ran this kit in separate weeks. The pre-coated plate format helped reduce operator-to-operator variation, and both runs produced similar quantitative patterns in our plasma/serum samples. The 100 µL volume was easy for our workflow, and the assay duration fit within a half-day. Overall, it supported reproducible IL‑23 testing in a busy lab environment.
  • Good long-term usability thanks to storage flexibility and 6‑month expiry
    We didn't have enough samples to run everything at once, so the six-month expiry and storage at 4°C or -20°C were important. The kit stayed consistent across staggered runs when we aliquoted standards and kept handling uniform. Quantitative results aligned with our expected biology, and the protocol was easy to follow. Great for labs collecting samples over time.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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