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Macrophage Interleukin 2 (IL2) Competition ELISA Kit (MTS-1123-HM68)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Interleukin 2 (IL2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Interleukin 2 (IL2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 2 (IL2)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 2

Synonyms

IL-2; TCGF; lymphokine

Background

This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM512	Goat		User optimized	Inquiry
MTS-1123-HM513	Cow		50-1000 pg/mL	Inquiry
MTS-1123-HM514	Sheep		50-1000 pg/mL	Inquiry
MTS-1123-HM515	Chicken		50-1000 pg/mL	Inquiry
MTS-1123-HM516	Pig		50-1000 pg/mL	Inquiry
MTS-1123-HM517	Mouse		50-1000 pg/mL	Inquiry
MTS-1123-HM518	Rat		50-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

When is competition ELISA preferable for IL-2 measurement compared with sandwich ELISA?

Competition ELISA can be useful when sample conditions vary widely or when you want a format that supports robust comparative quantitation in challenging matrices. IL-2 in co-culture can span a broad range, and competition format can be helpful for handling that variability-provided you validate dilution linearity early. If your samples are clean and you prioritize maximum sensitivity, sandwich ELISA is also a strong option; choice depends on your matrix and goals.

Can I use co-culture media containing serum, and what's your recommendation for dilution?

Serum-containing media can be used, but it may increase background depending on the matrix. We recommend clarifying samples, then running a small dilution series (e.g., two or three dilutions) in your first experiment to identify the best working dilution. Once you choose a dilution that yields stable, linear behavior, keep it consistent across your study to maintain comparability between conditions.

What controls should I include to convince reviewers the IL-2 numbers are solid?

A strong control set includes: (1) a standard curve on each plate, (2) a blank and a matrix control, (3) a known positive supernatant (activated T cells or a validated stimulant condition), and (4) spike-and-recovery checks in your matrix if you expect interference. Adding an internal reference sample across plates is also persuasive, because it demonstrates run-to-run stability beyond a single-day experiment.

Competition format handled variable co-culture matrices better than expected
We tested this kit with co-culture supernatants that were variable in composition and found it surprisingly robust once we optimized dilution. The data were consistent enough to support decision-making across multiple experimental batches. It wasn't completely "plug-and-play," but after a pilot run, the workflow was stable. It's a good option when your samples aren't perfectly uniform.
Helpful for comparing many conditions with confidence in relative IL-2 differences
Our project required comparing dozens of conditions rather than extracting one absolute value. The kit delivered consistent relative differences, which was exactly what we needed. We included a positive control and an internal reference aliquot, and that made the dataset reviewer-friendly. Once standardized, the assay became a dependable tool for ranking immunomodulatory conditions in our system.
Solid performance after implementing strict washing and timing consistency
We noticed early that small deviations in washing and incubation timing affected variability. After tightening our protocol execution-same operator steps, consistent wash cycles, careful plate sealing-replicates improved significantly. The kit then produced stable IL-2 comparisons across plates. If your lab can enforce good ELISA discipline, it's a reliable choice for high-throughput comparisons.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.