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Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 Protein ELISA Kit, Colorimetric (MTS-1123-HM41)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Specificity

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2)

Specification

Size

96 tests

Bioassay Target Name

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 2 (MGL2)

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

macrophage galactose N-acetyl-galactosamine specific lectin 2

Synonyms

CD301b

Background

Enables carbohydrate binding activity. Predicted to be active in external side of plasma membrane. Is expressed in ductus deferens; epididymis; ileum; prostate gland; and testis. Orthologous to human CLEC10A (C-type lectin domain containing 10A).

FAQs Customer Reviews Related Products

We measure lectin-related markers in macrophage polarization work. How specific is this kit for MGL2, and how do I avoid confusing signals from related lectins?

This kit is designed for quantitative measurement of MGL2 using a sandwich ELISA with a colorimetric readout. To minimize non-specific signals, we recommend careful blocking, thorough washing, and running matched negative controls (matrix-only and non-target samples). If you work with closely related lectin family members, you can validate specificity by using recombinant controls or knockdown/knockout samples in a small verification panel.

Can I use both cell lysates and supernatants, and what should I watch out for when switching matrices?

The product is intended for measurement in different sample types, and many users evaluate both lysates and supernatants depending on whether their biology is surface-associated, secreted, or shed forms. The key is consistency: keep lysis conditions mild, avoid interfering detergents when possible, and run dilution linearity checks for each matrix. If you share your buffer recipe, we can suggest adjustments to reduce background and improve curve performance.

What makes this kit "publication-ready" in terms of reproducibility and quality expectations?

For publication workflows, we recommend documenting your dilution strategy, running replicate standards and controls on every plate, and tracking intra- and inter-assay CVs across runs. This kit is positioned for quantitative readouts in a sandwich ELISA colorimetric format, which supports standard-curve based reporting when the assay is run within its validated range. We can advise on validation experiments (spike recovery and parallelism) so your methods and results are defensible in manuscripts and internal SOPs.

Strong quantitative signal with clean background in our hands for complex matrices and tight schedules
We used the MGL2 colorimetric kit for macrophage phenotype experiments and the plate readout was stable and easy to interpret. After a short optimization of sample dilution, we obtained a smooth standard curve and low background. The kit fit well into our standard ELISA schedule and did not require unusual equipment. Support was helpful in advising how to treat lysates versus supernatants, which reduced trial-and-error.
Good day-to-day usability and clear colorimetric endpoint for consistent, publication-ready reporting
The assay steps were familiar to anyone running sandwich ELISAs, and the colorimetric endpoint made it convenient for routine testing. We compared stimulated versus unstimulated macrophage conditions and saw changes consistent with our expectations. The supplier's recommendations on wash rigor and blocking were practical and improved our signal-to-noise. We would recommend it to labs that need quantitative lectin marker measurements without complex instrumentation.
Dependable kit for macrophage marker profiling panels for macrophage research workflows
We incorporate MGL2 into a broader marker panel, and this kit integrated smoothly into our workflow. The quantitative nature helped us normalize across batches and compare biological replicates with confidence. We appreciated that the supplier responded quickly to questions about matrix effects and offered a reasonable validation approach rather than generic advice. Overall, it's a dependable kit for macrophage profiling where consistent ELISA performance matters.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.