Macrophage Chemokine Ligand 17 (CCL17) ELISA Kit, qPCR (MTS-1123-HM93)

Combining ELISA and qPCR allows you to capture two complementary biological layers: secreted CCL17 protein levels in the supernatant and transcriptional regulation in the cells. This is particularly useful when treatments alter secretion dynamics differently than gene expression. With paired measurements from matched samples, you can present a clearer mechanism and reduce reviewer concerns about relying on only one readout. We can also advise on sampling timing to avoid missing transient expression peaks.

Yes, it can be used across donors and batches, but you should plan controls carefully. We recommend including a pooled reference sample that appears on every plate, using consistent dilution rules, and pre-defining replicate structure. For qPCR, stable housekeeping genes and consistent RNA quality metrics are essential. If you share your study design (donor count, time points, treatments), we can propose a practical control framework to keep comparisons defensible.

Start with a pilot run using a broad dilution series and include negative/blank controls and a mid-range positive control. If signals cluster near background, we can suggest concentrating supernatants (when appropriate) or adjusting sample preparation to preserve analyte integrity. For qPCR, increasing RNA input within recommended ranges and optimizing reverse transcription can help detect subtle induction. Our technical team can review your expected biology and recommend a realistic sensitivity strategy.