Macrophage Chemokine Ligand 9 (CXCL9) ELISA Kit, Colorimetric (MTS-1123-HM110)

This sandwich, colorimetric ELISA is suitable for common research matrices such as cell culture supernatants, serum, plasma, and clarified tissue or cell lysates, provided the matrix is properly prepared. For best accuracy, centrifuge samples to remove cells and particulates, and avoid hemolyzed or lipemic specimens when possible because they can increase background absorbance. If you work with serum/plasma, use consistent anticoagulants across the study and run a small dilution series first to ensure readings fall within the standard curve. For supernatants, filter or spin down debris and consider adding protease inhibitors during collection if CXCL9 degradation is a concern. Always equilibrate reagents to room temperature and mix gently to prevent bubble formation that can distort OD values.

The kit is designed for quantitative CXCL9 measurement using a two‑antibody sandwich format, which generally offers strong specificity and sensitivity for complex biological samples. If you expect low CXCL9 levels, start by running the provided standards carefully and verifying that the standard curve is smooth and monotonic. Then test a few representative samples at multiple dilutions (for example, neat, 1:2, and 1:5) to see where they land relative to the curve. If results cluster near the blank, optimize sample handling to reduce loss-use low‑bind tubes, minimize freeze-thaw cycles, and keep samples cold during processing. You can also increase signal quality by following wash steps rigorously and using calibrated pipettes to reduce well‑to‑well variation. If your expected concentrations are consistently below the usable range, consider alternative higher‑sensitivity formats such as qPCR‑based immunoassays.

A standard colorimetric sandwich ELISA workflow typically completes in about three hours from sample loading to final reading, depending on your incubation choices and wash routine. If you plan to split the kit, reseal unused strips immediately with the desiccant to protect coating integrity, and store them as instructed in the manual. Reagents should be kept tightly capped at recommended temperatures, commonly refrigerated, and standards or sensitive components should be aliquoted if repeated use is expected. Avoid repeated freeze-thaw cycles because antibody activity and standard stability can decline over time. Before each run, allow components to equilibrate to room temperature and mix gently; do not vortex foaming solutions. Finally, record lot numbers and run controls each time to maintain continuity across multiple assay days.