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Macrophage Chemokine Ligand 1 (CCL1) ELISA Kit, Colorimetric (MTS-1123-HM88)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Chemokine Ligand 1 (CCL1) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 1 (CCL1)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 1 (CCL1)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 1

Synonyms

P500; SISe; TCA3; I-309; SCYA1

Background

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM664	Human		312 pg/mL-5000 pg/mL	Inquiry
MTS-1123-HM665	Mouse		7.8 pg/mL-500 pg/mL	Inquiry
MTS-1123-HM666	Rat		62.5 pg/mL-4000 pg/mL	Inquiry
MTS-1123-HM667	Human		15.6-1000 pg/mL	Inquiry
MTS-1123-HM668	Mouse		30-8000 pg/mL	Inquiry
MTS-1123-HM669	Rabbit		25-500 pg/mL	Inquiry
MTS-1123-HM670	Rat		25-500 pg/mL	Inquiry
MTS-1123-HM671	Dog		25-500 pg/mL	Inquiry
MTS-1123-HM672	Guinea Pig		25-500 pg/mL	Inquiry
MTS-1123-HM673	Monkey		25-500 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is this kit suitable for standard colorimetric plate-reader workflows in a busy immunology lab?

Yes-this product is positioned as a colorimetric ELISA kit for CCL1, which typically aligns well with standard plate readers used in immunology labs. For best throughput, plan your plate map in advance, run duplicates, and include an internal control sample to monitor plate-to-plate drift. A brief pilot to identify the best dilution for your macrophage media will reduce repeat runs.

We want to measure CCL1 changes after macrophage polarization. What controls are most important?

Include untreated controls, vehicle controls (if applicable), and a positive control condition known to upregulate chemokines. Add spike-in recovery for at least a few representative samples to confirm the matrix does not suppress signal. Also, keep macrophage density, stimulation timing, and collection method consistent-those variables often drive more variation than the ELISA itself in polarization studies.

How can I avoid false differences caused by different serum percentages or supplements in culture media?

Media composition can strongly influence background and recovery. Ideally, keep serum percentage and supplements identical across conditions when comparing CCL1 output. If you must compare across different media, validate each matrix separately with dilution linearity and spike recovery, and avoid drawing hard conclusions from small differences. Using matrix-matched blanks and a consistent internal control sample helps you separate true biology from media effects.

Good colorimetric workflow for CCL1 monitoring in macrophage studies
We used this kit to monitor CCL1 changes during macrophage polarization experiments. The assay fit our standard plate reader setup and produced consistent trends once we selected the right sample dilution. The biggest improvement came from adding spike-in recovery checks in our macrophage media, which boosted confidence. After optimization, the results were reproducible and easy to integrate with our broader cytokine panel.
Clear differences between conditions after we standardized collection timing
Initially we saw variability, but it turned out to be sample collection timing and handling rather than the assay. After standardizing when we harvested supernatants and clarifying samples consistently, the readout stabilized. The kit then showed clear differences across our experimental groups and reduced the need for re-runs. It's a dependable choice if you approach it with disciplined sample handling.
Reliable for comparative studies; recommend a small pilot first
For our purposes, ranking conditions was more important than claiming absolute concentrations. This kit performed well for comparative analysis, especially once we ran a pilot dilution series to ensure our samples fell within the working range. With duplicates and an internal plate control, we obtained stable results across multiple experiments and felt confident using it for decision-making.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.