Macrophage Chemokine Ligand 18 (CCL18) ELISA Kit, Colorimetric (MTS-1123-HM34)

This kit is specified for cell culture supernatant, cell lysate, plasma, serum, and tissue homogenate. For tissue homogenates, the key is clean preparation: clarify by centrifugation, avoid harsh detergents that can affect binding, and test 2-3 dilutions to reduce matrix effects. The kit is described as a sandwich ELISA with colorimetric detection for quantitative measurement, so clean matrix handling and curve-fitting are the biggest drivers of reliable quantitation.

The specification lists an assay time of ~3 hours and a sample volume of 100 μL, and it's provided in a 96-test format with a pre-coated plate. Operationally, this means you can run the assay comfortably within a standard workday and plan sample volume accordingly (including duplicates and potential dilution repeats). If samples are limited, prioritize a pilot dilution first to avoid reruns.

The kit lists storage at 4°C and -20°C with an expiry of 6 months (refer to protocol guidance for component-specific handling). In practice, stability improves if you aliquot sensitive reagents upon first thaw, minimize freeze-thaw cycles, and keep a consistent equilibration routine (bringing components to room temperature uniformly before use). Also, record incubation timing carefully-timing drift is a common cause of long-term variability.