Human M2 Macrophage Differentiation Kit (MTS-1123-HM121)

The kit is designed around harvesting CD14+ monocytes from peripheral blood mononuclear cells (PBMCs) and driving ex vivo differentiation to provide an abundant source of M2 macrophages for downstream studies. A key benefit is operational simplicity: it uses validated, straightforward procedures and does not require specialized instrumentation. Practically, that means most standard cell culture labs can implement it with common equipment (biosafety cabinet, incubator, centrifuge). If you start with PBMCs, you can incorporate your preferred CD14+ enrichment approach, then follow the kit's differentiation steps to standardize polarization outcomes.

For M2 confirmation, we recommend a multi-layer validation plan: (1) surface markers commonly associated with M2-like macrophages (e.g., CD206, CD163) measured by flow cytometry; (2) cytokine profile checks (e.g., higher IL-10 relative to pro-inflammatory cytokines under relevant stimulation); and (3) functional assays aligned to your biology (wound-healing migration cues, phagocytosis changes, or T-cell modulation readouts). Since donor variability can be substantial, build acceptance criteria (marker thresholds and morphology notes) and always include an M0-like baseline and a pro-inflammatory comparator. This approach helps you interpret donor effects versus kit performance and improves reproducibility across studies.

The kit specification lists a storage temperature of -20 °C and an expiry date of 3 months, with storage guidance referencing the protocol. To support staggered experiments, plan your run calendar so all differentiations occur within the shelf-life window and minimize repeated freeze-thaw exposure by aliquoting components when permitted by the protocol. Also, standardize your monocyte input (cell number, viability, and isolation method) and keep a small batch of QC readouts (marker flow panels or qPCR checks) so you can confirm lot-to-lot and time-based consistency. If your timeline is longer than three months, consider ordering in phases.