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Macrophage Chemokine Ligand 4-Like 1 (CCL4L1) Competition ELISA Kit (MTS-1123-HM21)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Chemokine Ligand 4-Like 1 (CCL4L1) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 4-Like 1 (CCL4L1)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 4-Like 1 (CCL4L1)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 4 like 1

Synonyms

LAG1; CCL4L; LAG-1; SCYA4L; AT744.2; SCYA4L1; SCYA4L2; MIP-1-beta

Background

This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this family member is similar to the chemokine (C-C motif) ligand 4 product, which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals, where most individuals have one to five copies. Alternative splicing of this gene results in multiple transcript variants.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM212	Pig		User optimized	Inquiry
MTS-1123-HM213	Goat		User optimized	Inquiry
MTS-1123-HM214	Cow		User optimized	Inquiry
MTS-1123-HM215	Chicken		User optimized	Inquiry
MTS-1123-HM216	Sheep		User optimized	Inquiry

FAQs Customer Reviews Related Products

When would a competition ELISA be preferable for CCL4L1 compared with a sandwich ELISA?

A competition ELISA can be preferable when antibody-pairing for a sandwich format is challenging, when the antigen has limited accessible epitopes, or when sample conditions might block capture/detection simultaneously. In competition assays, sample antigen competes with a labeled or plate-bound antigen for antibody binding, which can sometimes tolerate certain matrices better. It's also useful when you want to compare relative antigen levels across groups with consistent assay conditions. The tradeoff is that the signal is inversely proportional to analyte concentration, so careful curve fitting and consistent handling are essential.

The signal goes down when concentration goes up-how do I avoid misinterpreting results?

That inverse relationship is normal in competition ELISA. The best practice is to rely strictly on the standard curve and avoid interpreting raw absorbance as "higher means more." Use the recommended curve model, keep incubation times identical across wells, and run samples in duplicates or triplicates. Serially dilute your samples to ensure they fall within the quantifiable range of the curve; if multiple dilutions converge on the same calculated concentration, that strongly supports accuracy. Also, avoid edge effects by equilibrating plates and reagents to temperature and sealing during incubations.

Can I compare results from this competition kit directly to another lab's sandwich ELISA values?

Direct comparison is not recommended unless both assays are harmonized with the same reference standard, calibration approach, and sample handling pipeline. Competition and sandwich ELISAs can yield different absolute values due to differences in antibody specificity, epitope recognition, and curve fitting. If cross-lab comparability matters, we suggest running a shared panel of samples and a shared reference material on both assays, then establishing a conversion or concordance relationship. For internal studies, consistency within one method is typically more important than matching another assay's absolute scale.

Competition format handled tricky matrices better than our previous sandwich assay
We switched to the competition kit after seeing non-parallel dilution behavior in a sandwich ELISA with complex tissue homogenates. With this competition format, our dilution series behaved more predictably, and the curve fitting produced stable concentrations. The inverse signal took one run to get used to, but once we focused on calculated values rather than absorbance, it was fine. Overall, it improved confidence in quantifying CCL4L1 in challenging sample backgrounds.
Accurate results after careful timing, sealing plates, and strict standard curve use
This kit rewards disciplined technique. When we kept incubation times consistent, used a plate sealer, and prepared standards carefully, the assay became very repeatable. We ran duplicates plus a dilution check for each sample and got close agreement between dilutions, which was reassuring. The reagents arrived well packaged and labels were clear. It's a good option when you need a robust ELISA readout but your sample matrix can interfere with typical sandwich capture steps.
Good sensitivity for relative comparisons across treatment groups in one study
We used the kit to compare CCL4L1 changes across macrophage stimulation conditions. The assay sensitivity was sufficient for our experimental ranges, and the performance was consistent across plates when we included the same internal control sample each time. The only caution is training new users on the "lower signal means higher concentration" concept. Once the team aligned on data processing, results were consistent and supported our conclusions.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.