Macrophage Chemokine Ligand 20 (CCL20) ELISA Kit, Colorimetric (MTS-1123-HM28)

Yes, inflammatory samples are common use cases for CCL20 measurement, but they can increase nonspecific background. To manage this, use appropriate sample dilution, include matrix blanks, and validate dilution linearity. If plasma/serum background is high, consider higher dilution and ensure consistent blocking and washing steps. Avoid letting the plate dry between washes, and use fresh wash buffer. It also helps to run spike-and-recovery tests to confirm that your matrix is not suppressing or inflating signal. With these controls, colorimetric ELISA can produce robust quantitative data.

For longitudinal studies, include a repeated internal control sample on every plate to track drift over time. Keep the same standard preparation approach each run and avoid mixing lots or substituting components mid-study. Run duplicates for all samples and repeat any that fall outside the curve range. Randomize sample placement to reduce edge effects, and document incubation times and temperatures. If multiple operators are involved, standardize the workflow with a checklist. These steps help ensure that time-point differences reflect biology rather than plate-to-plate or operator variability.

The most practical approach is dilution parallelism plus spike-and-recovery. Run at least two dilutions of representative samples; calculated concentrations should agree within an acceptable range. Then spike known antigen into your matrix and measure recovery; poor recovery suggests interference. Ensure your sample prep is consistent-especially for tissue homogenates where debris and proteases can affect results. Using protease inhibitors and clarifying samples can help. Finally, compare results with an orthogonal method for a subset of samples if the decision is high-impact.