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Macrophage Chemokine Ligand 24 (CCL24) ELISA Kit, qPCR (MTS-1123-HM95)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 24 (CCL24) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 24 (CCL24)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 24 (CCL24)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 24

Synonyms

Ckb-6; MPIF2; MPIF-2; SCYA24

Background

This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. This protein also has antimicrobial activity, displaying an antibacterial effect on S. pneumoniae, S. aureus, Non-typeable H. influenzae, and P. aeruginosa. Finally, the protein is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM904	Mouse	0.4 pg/mL		Inquiry
MTS-1123-HM905	Human	0.2 pg/mL		Inquiry

FAQs Customer Reviews Related Products

Can this kit help distinguish whether CCL24 changes are driven by transcription or secretion dynamics?

Yes, that is one of the key advantages of combining ELISA with qPCR. You can measure extracellular CCL24 protein and compare it with intracellular mRNA changes from matched samples. This is particularly useful when treatments alter secretion pathways, protein stability, or feedback signaling. By presenting both readouts, you can better support mechanistic claims and reduce ambiguity when protein and mRNA levels do not track perfectly.

What's the recommended workflow if I want to minimize sample handling while collecting both ELISA and qPCR data?

A streamlined approach is to collect supernatant for ELISA first, then immediately lyse or stabilize the corresponding cells for RNA extraction. Consistent timing is important so both readouts reflect the same biological state. We also recommend planning time points in advance and freezing aliquots to avoid repeated freeze-thaw cycles. If you share your stimulation duration and sample count, we can suggest a practical batch-processing schedule.

How should I troubleshoot if qPCR shows strong induction but ELISA shows only minor protein change?

This pattern can occur due to post-transcriptional regulation, delayed secretion, protein degradation, or assay-range issues. First, verify ELISA dilution and ensure samples fall in the linear range; then consider collecting additional time points to capture secretion lag. For qPCR, confirm housekeeping gene stability and RNA quality. If you share your time points and treatment type, we can propose targeted checks to determine whether the discrepancy is biological or technical.

Great for mechanistic storytelling by pairing ELISA and qPCR data
We used this kit to strengthen our interpretation of macrophage responses, and the combined readouts were genuinely helpful. In one condition, qPCR showed early mRNA induction while protein secretion increased later, and having both datasets made the story clearer. The workflow was manageable with good planning, and once we standardized timing, results were reproducible. It saved us from designing a separate qPCR approach from scratch.
Efficient workflow for screening plus validation in the same study
We ran ELISA screening across multiple treatments and then used qPCR on selected conditions to validate regulation. This reduced overall effort and improved the quality of our conclusions. The ELISA readout was consistent after we locked in a dilution, and the qPCR pairing helped explain borderline ELISA changes. Overall, it supported a clean experimental pipeline from discovery to mechanistic validation.
Good support and guidance on controlling variability across plates and runs
We appreciated the vendor's suggestions for adding a pooled reference control and defining acceptance criteria before starting a multi-week study. That advice helped us reduce variability and avoid re-running plates. The combined protein + mRNA approach also improved how we communicated results internally. If you're doing macrophage chemokine profiling and want both secretion and transcription information, this kit format is practical.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.