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Macrophage Inflammatory Protein Related Protein 1 (MRP1) ELISA Kit, Colorimetric (MTS-1123-HM43)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Macrophage Inflammatory Protein Related Protein 1 in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Lysate, Plasma, Serum, Tissue Homogenate

Specificity

Macrophage Inflammatory Protein Related Protein 1

Specification

Size

96 tests

Bioassay Target Name

Macrophage Inflammatory Protein Related Protein 1

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM332	Human		31.2 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM333	Mouse		31.2 pg/mL-2000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

We track inflammatory markers in macrophage activation. How quantitative is this kit, and what's the best way to ensure accurate plate-to-plate comparison?

This is a sandwich ELISA kit designed for quantitative measurement of MRP1 with a colorimetric method. For plate-to-plate comparability, we recommend using the same sample dilution scheme, running a full standard curve on every plate, and including an internal control sample (a pooled lysate/supernatant aliquot) to monitor drift. Recording incubation timing and wash consistency is also essential for stable quantification across runs.

If my samples show high background due to serum components, what troubleshooting steps do you recommend before repeating the entire assay?

High background is often addressed by optimizing dilution, increasing wash stringency, and ensuring blockers are fresh and compatible with your matrix. Since this kit is intended for measurement across different sample types, a small dilution gradient typically reveals whether matrix effects are driving the noise. We also suggest checking hemolysis/lipemia and clarifying samples thoroughly. If you share OD patterns and control performance, we can pinpoint likely causes quickly.

Can this kit support macrophage polarization studies (M1 vs M2) and drug screening, or is it mainly for baseline detection?

Quantitative ELISA is commonly used as part of macrophage profiling and screening workflows, especially when you need objective comparisons between conditions. This kit can support M1/M2-related studies by providing protein-level changes that complement gene expression or surface marker data. For drug screening, it's most useful when paired with appropriate controls and a predefined acceptance criterion (CV limits, curve fit quality) so results are decision-ready.

Reliable quantitative ELISA for inflammatory profiling panels for consistent, publication-ready reporting
We added the MRP1 kit to an inflammatory marker panel and it performed consistently across several macrophage activation experiments. The colorimetric readout was stable, and with a dilution pilot we minimized serum-driven background. The kit was easy to integrate into our weekly workflow, and the supplier's troubleshooting tips were actionable rather than generic. It's a dependable tool for quantitative profiling when you need comparable results across plates.
Good signal-to-noise after minor optimization of washing for macrophage research workflows
Our first run had slightly elevated background, but after tightening wash steps and using consistent timing, the assay became much cleaner. The standard curve behaved predictably and replicate variability was low. We used the kit for stimulated vs control macrophage conditions and observed differences matching our expectations. Overall, it's a solid quantitative kit, especially if you follow disciplined ELISA technique and document conditions carefully.
Helpful vendor support and consistent data across batches in our inflammation assay pipeline
We were concerned about batch-to-batch drift because our study spans multiple months. The supplier suggested adding a pooled internal control sample to every plate, which improved our confidence in cross-run comparisons. The kit delivered clear quantitative separation for our treatment groups and fit our existing plate-reader pipeline. Support was responsive and practical, making this a good choice for longer studies that require reproducibility.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.