Macrophage Chemokine Ligand 3 (CCL3) ELISA Kit, Colorimetric (MTS-1123-HM25)

In most cases, yes, but media composition can affect background and apparent recovery. Supplements like serum, high protein additives, or certain buffer components can increase nonspecific signal or interfere with antibody binding. We recommend using media-only controls, and if possible, collecting supernatants in consistent formulations across groups. Validate by running a dilution series and checking whether calculated concentration remains stable across dilutions. If not, a higher dilution or sample cleanup may be required. Keeping wash steps consistent and avoiding plate drying will also help maintain low background.

Near the low end, replicate strategy and signal stability become crucial. Run samples in triplicate when feasible, and include multiple low-concentration standards to anchor the curve in that region. Avoid long pauses during incubation or development steps, because small timing differences matter more when signals are weak. Make sure your plate reader settings are consistent and that you read promptly once the reaction reaches the recommended window. If low-level quantification is critical, confirm with an orthogonal method (e.g., another ELISA format or gene expression) to support conclusions.

Yes, and it's often preferable. Place stimulated and unstimulated groups on the same plate to minimize inter-plate variability. Randomize sample placement to reduce edge effects, include the full standard curve, and add at least one shared internal control sample repeated across plates if multiple plates are needed. Use the same reagent preparation, incubation times, and wash procedure throughout. If you must split across plates, analyze data with plate-based normalization using the internal control so comparisons remain robust.