Human Macrophage Polarization Markers qPCR Array Kit (MTS-1123-HM124)

This kit is designed for gene expression profiling of 40 marker genes involved in macrophage polarization, supporting studies on innate immune regulation and homeostasis. Instead of relying on one or two markers, you can evaluate multi-gene patterns that reflect mixed or transitional phenotypes, which are common in real biological systems (tumor microenvironments, chronic inflammation, biomaterials). For best interpretation, pair the array results with a small set of functional assays (cytokine release, phagocytosis, antigen presentation proxies) and include a consistent reference condition (e.g., M0 baseline) so you can interpret shifts as a polarization "direction" rather than a binary label.

The specification lists the detection method as qPCR, with storage at 4 °C and -20 °C (see protocol for details). To protect qPCR quality, use RNase-free technique throughout, assess RNA integrity (at least by A260/280 and a quick gel or fragment analysis if available), and keep reverse transcription timing consistent across samples. We also recommend including no-RT and no-template controls, plus one inter-run calibrator sample if you plan multiple plates. Finally, avoid repeated freeze-thaw cycles of sensitive components by aliquoting where allowed, and document Ct acceptance thresholds for internal QC.

With limited material, the key is RNA quality and consistent input rather than sheer quantity. You can often succeed by concentrating RNA, using high-efficiency reverse transcription, and keeping technical replicates focused on a subset of critical samples while prioritizing biological replicates. For analysis, use a clearly defined housekeeping strategy, apply ΔΔCt with an explicit reference condition, and pre-register criteria for calling genes "changed" (e.g., fold-change plus Ct reliability cutoffs). Visualization like heatmaps and pathway grouping of the 40 markers can make the narrative clearer. Reviewers also value cross-validation: confirm 3-5 key genes with independent primers or orthogonal protein assays.