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Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) Competition ELISA Kit (MTS-1123-HM55)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

Granulocyte-Macrophage Colony-Stimulating Factor 2 (CSF2)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Synonyms

CSF; GMCSF

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM398	Pig		User optimized	Inquiry
MTS-1123-HM399	Cow		User optimized	Inquiry
MTS-1123-HM400	Goat		User optimized	Inquiry
MTS-1123-HM401	Chicken		User optimized	Inquiry
MTS-1123-HM402	Sheep		User optimized	Inquiry
MTS-1123-HM403	Human		5.0-100 U/L	Inquiry

FAQs Customer Reviews Related Products

When is competitive CSF2 detection preferable to sandwich CSF2 detection?

Competitive assays can be attractive when you expect a broad range of cytokine levels, when sample matrix is complex, or when competitive format better matches available antibody performance. If your samples swing from very low to very high CSF2, competition can help avoid saturation once the dilution factor is optimized. We recommend a quick pilot dilution series to establish a stable operating point before scaling.

Does competitive ELISA take longer or require more complex data analysis?

The hands-on workflow is similar to other ELISAs, but interpretation differs because signal often decreases as analyte increases. Data analysis typically uses a standard curve and curve fitting like other immunoassays; the key is consistent plate setup and correct curve direction. We can provide guidance on plotting, control placement, and QC checks so results remain easy to review and defensible.

How do I ensure my competition curve isn't distorted by pipetting variability?

Competitive formats can be sensitive to small pipetting errors, especially in standards. We recommend careful serial dilutions with thorough mixing, using calibrated pipettes, and running standards in duplicates or triplicates. Standardizing incubation times and wash steps also matters. If you run many plates, include the same QC sample on every plate to detect drift early and correct workflow issues before they compound.

Helpful competitive option for wide-range CSF2 measurements across donors
We had highly variable CSF2 release across donors and conditions. The competitive format worked well after we did a small pilot dilution curve and standardized our plate timing. Results were reproducible enough to rank donors and select outliers for follow-up. Support also clarified curve interpretation for new team members, which helped us avoid early mistakes and reduce reruns.
Good kit for screening; consistent once QC and dilution are standardized
We use this assay primarily for screening many samples quickly rather than ultra-precise absolute quantification. After adopting a fixed dilution factor and including a pooled QC sample on each plate, variability decreased and trends were stable. It's a practical tool for prioritizing conditions and deciding which samples merit deeper validation by other methods.
Clear workflow and stable results after tightening pipetting discipline
The biggest improvement came from better standard preparation and consistent mixing. Once we implemented a strict dilution SOP and repeated standards, the curves looked much cleaner. The kit then produced consistent outputs across plates, which was essential for our multi-week study. Overall, a reliable competitive ELISA when executed with good technique.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.