Macrophage Chemokine Ligand 16 (CXCL16) Competition ELISA Kit (MTS-1123-HM119)

This kit uses a competition ELISA method with colorimetric detection for quantitative CXCL16 measurement. Competitive ELISA can be advantageous when epitope accessibility is variable, when you want an alternative format to cross-check sandwich ELISA results, or when your matrix complicates capture/detection antibody pairing. The tradeoff is that competitive assays often require extra care in curve interpretation because signal decreases as analyte increases. If you prioritize orthogonal confirmation or encounter sandwich-format interference, the competitive kit can be a strong complement rather than a replacement.

The kit lists compatibility with cell culture supernatant, plasma, serum, and tissue homogenate. For macrophage-conditioned media, clarification (spin down debris) and consistent dilution are usually sufficient. For tissue homogenates, standardize the homogenization buffer and protein normalization approach across samples to reduce variability. We also recommend running dilution linearity to ensure the homogenate matrix does not distort the competitive curve. Keeping sample handling consistent-especially freeze-thaw cycles-will help your quantitative results remain interpretable across batches.

Key operational specs on the product page include 1.5 hours total assay time, 100 μL sample volume per well, and a pre-coated plate format, which streamlines setup. The listed kit size is 96 tests, storage is 4°C, and the stated expiry is 6 months (with storage guidance to follow the protocol). The page also clearly notes the kit is for research use only and not for human/animal use. These details make it suitable for teams needing fast turnaround with predictable logistics.