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Macrophage TNF alpha Competition ELISA Kit (MTS-1123-HM62)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Tumor Necrosis Factor alpha (TNF alpha) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Plasma, Serum, Tissue Homogenate

Specificity

Tumor Necrosis Factor alpha (TNF alpha)

Specification

Size

96 tests

Sample Volume

50 μL

Assay Time

1 - 4.5 h

Plate

Pre-coated

Bioassay Target Name

Tumor Necrosis Factor alpha (TNF alpha)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Synonyms

TNF; TNF-α

Background

Tumor necrosis factor (TNF, cachexin, or cachectin; formerly known as tumor necrosis factor alpha or TNF-α) is an adipokine and a cytokine. TNF is a member of the TNF superfamily, which consists of various transmembrane proteins with a homologous TNF domain.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM447	Chicken		0.27 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM448	Cow		0.1-20 ng/mL	Inquiry
MTS-1123-HM449	Sheep		3.125-50 pg/mL	Inquiry
MTS-1123-HM450	Fish		125-5000 pg/mL	Inquiry
MTS-1123-HM451	Mouse		50-1000 pg/mL	Inquiry
MTS-1123-HM452	Pig		50-1000 pg/mL	Inquiry
MTS-1123-HM453	Goat		50-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Why should I choose a competition ELISA for TNF-α instead of a sandwich ELISA?

Competition ELISA is often preferred when the target is small, when epitope accessibility varies, or when you want a format that can be more tolerant of certain sample conditions. In this kit, TNF-α is measured quantitatively with a competition ELISA and colorimetric detection. It can be a good choice when you need a reliable comparative readout across many samples, especially if you've encountered sandwich-format constraints.

What sample types are validated, and can I use macrophage lysates?

The listed sample types include plasma, serum, and tissue homogenate. For macrophage experiments, many customers use supernatants, but if you specifically want lysates, we recommend a quick pilot validation: check dilution linearity and spike recovery to confirm the lysate buffer doesn't interfere with binding. If detergents are present, reducing detergent concentration or switching buffers can improve performance.

How long does the assay take and what volume do I need per well?

The kit is designed to be efficient: typical total assay time is about 1-4.5 hours depending on incubation choices, and the sample volume is 50 µL per well on a pre-coated plate. This makes it suitable for studies where sample volume is limited or when you need same-day results for iterative experimental decisions.

Competition format gave stable TNF-α quantification across complex samples
We chose the competition ELISA specifically because some of our matrices were less "clean" than cell culture supernatant. The kit handled the workflow smoothly, and the timing was manageable within a workday. We liked that the plate is pre-coated and the sample volume is modest, which helped when sample was limited. Data were consistent across replicates, giving us confidence in comparative TNF-α trends.
Fast turnaround with predictable performance and easy plate handling
The assay fit into a tight schedule and we consistently finished within the stated range, depending on incubation time. The pre-coated format reduced variability and made setup quicker. We also appreciated the clear sample type guidance (serum/plasma/tissue homogenate), which matched our translational workflow. Overall, it felt like a well-structured kit for routine TNF-α monitoring.
Good quantitative readout after we optimized dilution for tissue homogenates
Tissue homogenates can be messy, and we expected some trial-and-error. After testing a small dilution series, the results stabilized and the background dropped. Once optimized, the kit gave a clean dose-dependent signal that matched our inflammatory model. The 96-test format was also helpful because we could include controls and multiple dilutions without worrying about running out of wells.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.