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Macrophage TGF-beta (TGFβ) Competition ELISA Kit (MTS-1123-HM103)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of TGF-beta (TGFβ) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

TGF-beta (TGFβ)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Pre-coated

Bioassay Target Name

TGF-beta (TGFβ)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM709	Rabbit		100-2500 pg/mL	Inquiry
MTS-1123-HM710	Rat		50-1000 pg/mL	Inquiry
MTS-1123-HM711	Goat		50-1000 pg/mL	Inquiry
MTS-1123-HM712	Human		50-1000 pg/mL	Inquiry
MTS-1123-HM713	Monkey		50-1000 pg/mL	Inquiry
MTS-1123-HM714	Mouse		100-2500 pg/mL	Inquiry
MTS-1123-HM715	Guinea Pig		100-2500 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is the competition format advantageous for complex tissue samples?

Yes, the competitive format is beneficial when analyzing tissue homogenates or samples with high protein background. It reduces steric hindrance and nonspecific interference, improving specificity and measurement accuracy for TGF-beta.

Can this kit be used for moderate-throughput experiments?

The 96-well format allows simultaneous processing of multiple samples with a single standard curve. With consistent timing and washing technique, users can efficiently analyze mid-sized sample sets.

How should competitive assay data be analyzed?

Because signal intensity is inversely proportional to concentration, four-parameter logistic curve fitting is recommended. Proper standard preparation and replicate wells enhance quantitative accuracy.

Effective in complex matrices
We applied this kit to tissue homogenates and observed improved stability compared to sandwich assays. Background interference was reduced, and the standard curve remained consistent.
Reliable quantitative performance
The competitive format required careful curve interpretation, but once optimized, results were reproducible and clear across biological replicates.
Suitable for mechanistic studies
We used this kit in TGF-beta pathway analysis and obtained stable quantitative differences between treatment groups.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.