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Macrophage Chemokine Ligand 2 (CXCL2) ELISA Kit, Colorimetric (MTS-1123-HM38)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Chemokine Ligand 2 (CXCL2) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Lysate, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 2 (CXCL2)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 2 (CXCL2)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-X-C motif chemokine ligand 2

Synonyms

GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a

Background

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM311	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM312	Rat		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM313	Mouse		12.5 pg/mL-800 pg/mL	Inquiry
MTS-1123-HM314	Human		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM315	Human		3.12 pg/mL-200 pg/mL	Inquiry
MTS-1123-HM316	Mouse		0.02-1.0 ng/mL	Inquiry
MTS-1123-HM317	Rat		0.03-2.0 ng/mL	Inquiry
MTS-1123-HM318	Dog		25-500 pg/mL	Inquiry
MTS-1123-HM319	Monkey		25-500 pg/mL	Inquiry
MTS-1123-HM320	Rabbit		100-2500 pg/mL	Inquiry
MTS-1123-HM321	Guinea Pig		100-2500 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is this CXCL2 colorimetric kit quantitative and what ELISA format does it use?

The indexed product snippet describes it as a sandwich ELISA kit with colorimetric detection for quantitative measurement of CXCL2. For best quantitative performance, run a complete standard curve every plate, keep timing consistent, and verify that your samples fall within the linear range (often by testing two dilutions on the first run). This reduces reruns and improves confidence in reported concentrations.

I'm measuring CXCL2 after strong inflammatory stimulation-how do I avoid saturating the assay?

Strong stimulation can produce very high chemokine levels, so saturation is a real risk. The simplest prevention is planning dilutions proactively: run at least two dilutions per sample in your first experiment and choose the one that lands mid-curve. Also, keep the same sample handling across groups to avoid artificial differences caused by storage time or freeze-thaw. When you manage range carefully, quantitative comparisons become much more reliable.

Can I use this kit for both supernatants and serum, and what should I validate first?

The kit is presented as suitable for quantitative CXCL2 measurement in different sample types. Since matrices can behave differently, validate with a quick spike-and-recovery or dilution linearity check in each matrix you plan to publish. Often, serum requires higher dilution or additional clarification to reduce background. Once you confirm recovery and linearity, you can confidently compare within the same matrix across experimental groups.

Clean quantitative sandwich ELISA signal for CXCL2 in macrophage supernatants
We used this kit to quantify CXCL2 in stimulated macrophage-conditioned media. The colorimetric readout was clear and the standard curve fit nicely after we selected the right dilution. Our strongest conditions would have saturated without dilution optimization, so running two dilutions initially was essential. After that, the assay delivered stable quantitative results that aligned with our expected inflammatory phenotype changes across treatments.
Solid routine CXCL2 assay with minimal equipment needs and good repeatability
This kit fits a standard ELISA pipeline-plate reader only, no specialized instruments. Once we standardized incubation timing and used the same internal control sample across plates, repeatability improved significantly. For our purposes (comparing treatment groups), the quantitative output was consistent and analysis was straightforward. If you follow good dilution practices and keep sample handling consistent, it's a dependable CXCL2 measurement tool.
Works well for inflammatory panels when you need quantitative chemokine readouts
We run a panel of inflammatory markers and added CXCL2 using this colorimetric kit. The sandwich ELISA approach gave stable curves and good signal-to-background once we optimized dilution for serum. We recommend adding a pooled reference sample to each plate to track drift over time. Overall, it's been a practical addition to our panel and supports quantitative comparisons across cohorts.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.