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Macrophage Chemokine Ligand 5 (CCL5) ELISA Kit, Colorimetric (MTS-1123-HM85)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Chemokine Ligand 5 (CCL5) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 5 (CCL5)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

3 h

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 5 (CCL5)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 5

Synonyms

SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta

Background

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM645	Mouse		78 pg/mL-5000 pg/mL	Inquiry
MTS-1123-HM646	Human		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM647	Rat		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM648	Guinea Pig		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM649	Pig		3.12 ng/mL-200 ng/mL	Inquiry
MTS-1123-HM650	Mouse		15.6 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM651	Monkey		31.25 pg/mL-2000 pg/mL	Inquiry
MTS-1123-HM652	Chicken		15.625 pg/mL-1000 pg/mL	Inquiry
MTS-1123-HM653	Dog		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM654	Cat		0.7-200 pg/mL	Inquiry
MTS-1123-HM655	Rabbit		2.5-50 ng/mL	Inquiry
MTS-1123-HM656	Cow		78.125 pg/mL-5000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

Is this kit intended for sandwich ELISA quantification of CCL5, and what readout does it use?

Yes-this product is described as a sandwich ELISA kit for quantitative measurement of CCL5 using a colorimetric detection approach. Sandwich ELISAs are widely used for secreted chemokines like CCL5 because they typically provide high specificity through dual-antibody recognition. We recommend a pilot run to choose the right sample dilution and to confirm linearity in your macrophage media.

We see very high CCL5 after stimulation-how do we avoid saturating the assay?

High chemokine output is common under strong stimulation. The safest approach is to pre-test a few representative samples with serial dilutions, then select a dilution that places most samples in the mid-range of the curve. Keep dilution consistent across conditions to avoid bias. If your highest samples still saturate, re-run those at a higher dilution and apply the correct back-calculation.

How can I ensure the data are comparable across a multi-week study?

Use standardized collection times, consistent freezing/aliquoting practices, and include the same internal control sample on every plate. If your project spans multiple kit lots, consider purchasing enough for the full study or running a bridging plate to assess lot-to-lot continuity. This is especially important in macrophage experiments where day-to-day biological variability can otherwise obscure real differences.

Strong colorimetric sandwich ELISA performance for macrophage CCL5
We measured CCL5 in macrophage-conditioned media across several stimuli. After a short pilot to pick dilution factors, the assay produced consistent readings and clear differences across groups. The colorimetric format was easy for our team to run, and the results aligned with our expected biology. It became a reliable component of our cytokine/chemokine panel for ongoing experiments.
Good sensitivity after optimization; works well for screening conditions
Our primary use was screening compounds that modulate macrophage chemokine output. This kit gave clean, interpretable results once we standardized sample collection and implemented duplicate wells. We appreciated the straightforward readout and how easily it fit into our plate reader workflow. It's a good practical choice if you want consistent relative differences between conditions without heavy troubleshooting.
Clear protocol and reproducible results when you control timing carefully
We found that consistent incubation timing and thorough washing were the biggest factors affecting variability. Once those were standardized, the assay was very reproducible. It separated treated versus untreated macrophage groups clearly, and it was easy to train new lab members to run. Overall, it delivered dependable CCL5 quantification for our ongoing studies.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.