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Macrophage Chemokine Ligand 26 (CCL26) ELISA Kit, qPCR (MTS-1123-HM37)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 26 (CCL26) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sensitivity

2.8 pg/mL

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 26 (CCL26)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 26 (CCL26)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 26

Synonyms

IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha

Background

This gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for normal peripheral blood eosinophils and basophils. This protein also has antimicrobial activity, displaying an antibacterial effect on S. pneumoniae, S. aureus, Non-typeable H. influenzae, and P. aeruginosa. The product of this gene is one of three related chemokines that specifically activate chemokine receptor CCR3. This chemokine may contribute to the eosinophil accumulation in atopic diseases.

FAQs Customer Reviews Related Products

What are the key specs-reactivity, sensitivity, and sample types-for this CCL26 qPCR kit?

The page specifies Human reactivity, qPCR detection, and a sandwich ELISA workflow with semi-quantitative analysis. It lists 2.8 pg/mL sensitivity and sample types including cell culture supernatant, plasma, and serum. It also notes 96 tests and a 25 µL sample volume, with storage listed at 4°C, -20°C, and -80°C and a 6-month expiry.

We're short on sample-does the 25 µL volume mean I can run duplicates easily?

Yes, the listed 25 µL per well is helpful when sample volume is limited, especially if you need duplicates plus a couple of dilutions. In practice, you can plan a small pilot with two dilutions for a subset of samples, then lock in the best dilution for the full cohort. That strategy reduces reruns and preserves valuable sample. Also, keep a pooled reference control aliquot to strengthen plate-to-plate comparisons in semi-quantitative workflows.

What's the best way to use this semi-quantitative kit in a study that needs strong statistical comparisons?

For strong statistics, standardize everything upstream: sample collection timing, storage, and freeze-thaw behavior. Then, run duplicates, keep the same incubation timing across plates, and include a consistent reference sample (and ideally a small dilution series) on every plate to track drift. With semi-quantitative assays, these anchors let you compare groups confidently and reduce batch effects. If your endpoint demands absolute quantitation claims, confirm key findings with a fully quantitative assay.

Low sample volume requirement made CCL26 profiling much more feasible
We picked this kit largely because it only needs 25 µL per well, which is a big advantage for limited clinical serum. The assay gave stable relative differences between groups and the sensitivity listed on the page (2.8 pg/mL) matched our need to detect low signals. Using a pooled reference control each run improved consistency across plates. Overall, it's a strong option for semi-quantitative CCL26 comparisons in human samples.
Good sensitivity and clear performance once we standardized serum handling conditions
We were concerned about low-level CCL26 signals, but the kit sensitivity and workflow worked well once we optimized dilution. The main factor was keeping serum handling consistent: same processing time, same storage conditions, and minimal freeze-thaw. The semi-quantitative output was sufficient for our project because we focused on relative comparisons rather than absolute concentrations. It's a practical balance of sensitivity and sample conservation.
Strong semi-quant kit for screening cohorts before confirmatory quantitative assays
We used this kit as a cohort-screening step to identify samples with the strongest CCL26 changes. The 96-test format fit our throughput needs, and the storage flexibility (including -80°C) helped with staging the study over time. After ranking samples with this semi-quantitative approach, we ran a smaller confirmatory set with another method. This two-step workflow saved time and reduced reagent waste while keeping conclusions robust.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.