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Macrophage Interleukin 6 (IL6) Competition ELISA Kit (MTS-1123-HM74)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Interleukin 6 (IL6) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Serum, Tissue Homogenate

Specificity

Interleukin 6 (IL6)

Specification

Size

96 tests

Sample Volume

50 μL

Assay Time

1 - 4.5 h

Plate

Pre-coated

Bioassay Target Name

Interleukin 6 (IL6)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

interleukin 6

Synonyms

CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM572	Fish		12.5-200 pg/mL	Inquiry
MTS-1123-HM573	Cow		5-1000 pg/mL	Inquiry
MTS-1123-HM574	Sheep		2-80 pg/mL	Inquiry
MTS-1123-HM575	Pig		50-1000 pg/mL	Inquiry
MTS-1123-HM576	Goat		50-1000 pg/mL	Inquiry
MTS-1123-HM577	Human		50-1000 pg/mL	Inquiry
MTS-1123-HM578	Chicken		User optimized	Inquiry
MTS-1123-HM579	Mouse		50-1000 pg/mL	Inquiry

FAQs Customer Reviews Related Products

What are the key differences between this competition IL‑6 kit and a sandwich IL‑6 kit in daily use?

This product is a colorimetric, quantitative competition ELISA for IL‑6. Compared with sandwich assays, competition formats can be helpful in certain conditions where two-site binding is challenging. Operationally, you must be especially disciplined with dilution accuracy and mixing because the signal relationship is inverse with analyte amount. Replicates and consistent incubation timing are essential to reduce variability in competition curves.

What sample types and volumes are required? We have limited material.

The kit lists serum and tissue homogenate as sample types, and the sample volume is 50 µL per well, which is relatively sample-sparing. This is helpful when tissue yield is limited. We recommend running dilution checks for tissue homogenate due to viscosity and matrix effects, and clarifying samples to reduce nonspecific background that could compress your dynamic range.

How long does the assay take, and is the plate pre-coated?

The plate is pre-coated, and the assay time is stated as 1-4.5 hours depending on protocol steps. Pre-coated plates reduce variability and save prep time. For best throughput, prepare your standard dilution series carefully, use consistent wash technique, and keep plate handling uniform across wells-competition assays are sensitive to minor timing differences that can shift the curve.

Sample-sparing volume and stable performance for tissue homogenate applications
We liked that only 50 µL per well was needed, since our tissue homogenate samples were limited. The competition format required careful pipetting and consistent timing, but once optimized, the curve was stable and repeatable. It was a good fit for our IL‑6 profiling work where tissue sample conservation mattered. Pre-coated plates also reduced setup complexity.
Useful quantitative IL‑6 kit when sandwich binding was not ideal
In our hands, some treatment conditions created matrix effects that complicated sandwich ELISA interpretation. This competition ELISA provided an alternate quantitative approach and worked reliably after we standardized mixing and wash steps. The protocol fit within a workday, and we achieved consistent trends across replicates. It's a good option for teams comfortable with competition assay data interpretation.
Pre-coated convenience plus strong reproducibility after training new staff
We trained a newer technician on this kit. The pre-coated plate and clear kit structure helped reduce early errors, but we still emphasized dilution accuracy because competition assays are sensitive. After two practice runs, results became very consistent and correlated with our expected biological responses. Overall, it's reliable if users follow timing strictly and keep pipetting technique consistent.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.