Macrophage Colony Stimulating Factor 1 (M-CSF/CSF1) ELISA Kit, Colorimetric (MTS-1123-HM58)

Yes. M-CSF is commonly monitored in differentiation and polarization workflows, and a colorimetric sandwich ELISA is well-suited for routine measurement in supernatants. For best results, collect samples at consistent timepoints, clarify by centrifugation, and include a consistent QC sample on each plate. If your media contains supplements, we recommend matrix checks to confirm recovery and minimize background.

Longitudinal consistency comes from controls and standardization. Use the same incubation timing, the same curve-fitting method, and the same dilution strategy throughout the study. Include a bridging QC sample on every plate (ideally aliquoted and frozen once) to monitor drift. If you change lots or operators, run an overlap plate to quantify any shift before continuing.

In general, minimize freeze-thaw cycles by aliquoting samples, store at appropriate cold temperatures, and avoid prolonged time at room temperature. Clarify supernatants before freezing to reduce protease-rich debris. If you're comparing subtle differences, standardize collection and storage across groups. We can suggest a simple stability check by comparing freshly processed samples vs. frozen aliquots.