Online Inquiry

Macrophage Chemokine Ligand 1 (CCL1) Competition ELISA Kit (MTS-1123-HM89)

Datasheet

Overview

Description

Creative Biolabs provides competition ELISA kit for quantitative measurement of Chemokine Ligand 1 (CCL1) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Competition ELISA

Analytical Method

Quantitative

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Specificity

Chemokine Ligand 1 (CCL1)

Specification

Size

96 tests

Sample Volume

100 μL

Assay Time

1.5 h

Plate

Strips (12 x 8)

Bioassay Target Name

Chemokine Ligand 1 (CCL1)

Storage

4 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 1

Synonyms

P500; SISe; TCA3; I-309; SCYA1

Background

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM674	Pig		User optimized	Inquiry
MTS-1123-HM675	Human		250-5000 pg/mL	Inquiry
MTS-1123-HM676	Sheep		User optimized	Inquiry

FAQs Customer Reviews Related Products

What is the detection method and intended measurement type for this CCL1 kit?

This product is described as a competition ELISA kit for quantitative measurement of CCL1 using a colorimetric readout. Competition formats are commonly selected when assay design benefits from competitive binding behavior, and they can be useful across different sample contexts when validated with appropriate dilution and recovery checks.

How should I validate that my macrophage supernatant won't interfere with the competition assay?

Start with a dilution series of representative samples and confirm that calculated concentrations scale proportionally (dilution linearity). Next, perform spike-in recovery by adding a known amount of CCL1 standard into your matrix and verifying acceptable recovery. Include matrix blanks and run duplicates. These steps are especially important in competition ELISAs because matrix components can influence competitive binding and shift apparent concentrations.

Can this kit be used for both exploratory screening and more rigorous studies?

Yes, with the right structure. For exploratory screening, consistent dilution and duplicates are usually sufficient to rank conditions. For more rigorous studies, add replicate plates, internal controls across plates, and predefine acceptance criteria for CV and recovery. Also, keep macrophage culture conditions tightly controlled-cell density, stimulation time, and collection method-so the assay is measuring biology rather than process variation.

Quantitative competition ELISA that worked well after matrix validation steps
We used this kit for CCL1 quantification in macrophage supernatants. After running dilution linearity and spike recovery, the assay produced stable results that aligned with our expected treatment effects. The colorimetric readout was easy to implement, and the data were consistent between duplicates. It's a good choice if you're willing to do a small amount of upfront validation for your specific media conditions.
Good for comparing groups; best results came with strict timing control
Our results improved dramatically once we standardized incubation timing and washing technique. With those controls in place, the assay separated our macrophage polarization conditions clearly. The competition format handled our samples well, and we could run multiple batches without major drift by including an internal control sample on each plate. Overall, it supported our comparative analysis reliably.
Practical kit for macrophage chemokine profiling when you keep good controls
We added this CCL1 competition ELISA into a larger chemokine panel. It performed well for decision-making and trend analysis, especially when we kept sample handling consistent and avoided freeze-thaw cycles. We also found that including spike-in controls helped catch a media batch that caused lower recovery. With basic best practices, the kit delivered dependable quantitative information.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.