Macrophage Chemokine Ligand 10 (CXCL10) ELISA Kit, Colorimetric (MTS-1123-HM113)

This CXCL10 sandwich, colorimetric ELISA is intended for common matrices such as cell culture supernatants, serum, plasma, and clarified tissue or cell lysates, assuming samples are prepared cleanly. For serum/plasma, avoid hemolysis and lipemia because both can elevate baseline absorbance and compromise quantification. Centrifuge samples to remove debris, and if you are working with supernatants from activated macrophages, spin down any detached cells before loading. Run a dilution series to ensure measurements sit within the standard curve and to reduce matrix effects. Consistent anticoagulant choice (if plasma) and uniform collection times across groups will improve comparability. Finally, perform washes thoroughly and read the plate promptly after substrate development to prevent signal drift.

A valid run usually shows low blank absorbance, replicates with tight CVs, and a standard curve that progresses smoothly across concentrations with expected monotonic behavior. If the curve is uneven, first verify pipetting accuracy and mixing of standards-incomplete resuspension or serial dilution errors are common culprits. Next, check wash performance; residual liquid can dilute reagents and increase variability, while over‑aggressive washing can damage the coating. Ensure incubation times and temperatures are consistent across the plate, particularly if you load wells sequentially; a multichannel pipette helps reduce timing drift. Also confirm that the substrate is fresh, protected from light, and that the plate reader is calibrated at the correct wavelength. If matrix effects are suspected, additional sample dilution or using matrix‑matched diluent can often restore parallelism and improve curve fitting.

If you plan to run multiple plates over time, store the coated plate strips sealed with the provided desiccant to prevent moisture damage and loss of binding capacity. Keep reagents at the temperatures specified in the manual, typically refrigerated, and aliquot any standards or concentrated components that will be opened repeatedly to minimize contamination and degradation. Avoid repeated freeze-thaw cycles for sensitive proteins and antibodies, and label aliquots with date and lot number for traceability. Before each assay, allow components to equilibrate to room temperature and mix gently without foaming. We also recommend running the same internal control sample across sessions to monitor drift; this makes it easier to compare CXCL10 data across different experimental days and ensures confidence in longitudinal studies.