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Macrophage Stimulating 1 (MST1) ELISA Kit, Colorimetric (MTS-1123-HM10)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for quantitative measurement of Macrophage Stimulating 1 (MST1) in different sample types by colorimetric.

Applications

ELISA

Qualified With

Quality Certificate

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Analytical Method

Quantitative

Sample Type

Plasma, Serum, Tissue Homogenate

Specificity

Macrophage Stimulating 1 (MST1)

Specification

Size

96 tests

Sample Volume

100 μL

Plate

Pre-coated

Bioassay Target Name

Macrophage Stimulating 1 (MST1)

Storage

4 °C, -20 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

macrophage stimulating 1

Synonyms

MSP; HGFL; NF15S2; D3F15S2; DNF15S2

Background

The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.

Sub Cat	Reactivity	Sensitivity	Detection Range
MTS-1123-HM156	Mouse		3.125 ng/mL-200 ng/mL	Inquiry
MTS-1123-HM157	Human		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM158	Rat		0.15 ng/mL-10 ng/mL	Inquiry
MTS-1123-HM159	Pig		User optimized	Inquiry
MTS-1123-HM160	Rabbit		User optimized	Inquiry

FAQs Customer Reviews Related Products

Can I compare MST1 results across tissue homogenates and cell supernatants in the same study?

You can, but interpret carefully: matrices differ dramatically and can shift apparent recovery. For strong conclusions, validate each matrix separately with parallelism and recovery checks, then keep comparisons primarily within the same matrix type. If you must compare across matrices, use normalization strategies and include shared reference controls. This keeps your analysis grounded and avoids mistaking matrix effects for biological differences.

What's the most common cause of unexpectedly high background in colorimetric MST1 ELISA runs?

The most common causes are insufficient washing, overly long incubation with detection reagents, or sample matrix components that promote non-specific binding. To address this, increase wash rigor (without damaging the plate), ensure consistent aspiration, and avoid splashing between wells. Run matrix blanks to identify whether the background is sample-driven. If the issue is matrix-related, diluting samples or modifying sample preparation often improves specificity.

MST1 can be present in complex matrices. What's your recommended validation checklist before running a big cohort?

Before scaling, run a short validation: (1) dilution linearity/parallelism in your matrix, (2) spike-and-recovery in representative samples, (3) replicate precision (duplicate/triplicate), and (4) stability check for storage conditions (fresh vs frozen aliquots). Because the kit is designed for quantitative measurement via colorimetric sandwich ELISA, these steps help confirm that your matrix behaves similarly to standards and that results will be reliable across a cohort.

Quantitative MST1 measurement worked well after matrix validation steps
We ran this MST1 kit on both cell supernatants and a limited set of tissue homogenates. The key to success was doing parallelism and spike recovery before committing a full cohort. Once validated, the assay delivered consistent curves and clear group separation. The colorimetric readout is easy to implement, and the workflow matched standard sandwich ELISA procedures, which made it accessible to our whole team.
Practical kit for cohort studies once sample dilution is optimized
We initially struggled because several samples were too concentrated and saturated the curve. After selecting appropriate dilutions, the assay became reliable and our replicate CVs improved. The kit is straightforward and doesn't require specialized equipment, which is helpful for routine use. Overall it provided dependable quantitative results for MST1 in our macrophage-related study, especially after early optimization.
Low background and good repeatability when washing is consistent
In our hands, background stayed low as long as we used disciplined wash steps and consistent incubation times. We included a pooled reference sample and that improved plate-to-plate comparability. The kit worked smoothly on a standard plate reader and helped us quantify MST1 trends across treatment groups. I'd recommend avoiding multiple freeze-thaw cycles and running samples in duplicates for best precision.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.