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Macrophage Chemokine Ligand 13 (CCL13) ELISA Kit, qPCR (MTS-1123-HM101)

Datasheet

Overview

Description

Creative Biolabs provides sandwich ELISA kit for semi-quantitative measurement of Chemokine Ligand 13 (CCL13) in different sample types by qPCR.

Applications

ELISA

Qualified With

Quality Certificate

Reactivity

Human

Detection Method

qPCR

Method Type

Sandwich ELISA

Analytical Method

Semi-Quantitative

Sensitivity

0.3 pg/mL

Sample Type

Cell Culture Supernatant, Plasma, Serum

Specificity

Chemokine Ligand 13 (CCL13)

Specification

Size

96 tests

Sample Volume

25 µL

Plate

Pre-coated

Bioassay Target Name

Chemokine Ligand 13 (CCL13)

Storage

4 °C, -20 °C, -80 °C

Storage Comment

Reference to the protocol

Expiry Date

6 months

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.

Target Details

Full Name

C-C motif chemokine ligand 13

Synonyms

NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13

Background

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis.

FAQs Customer Reviews Related Products

Can this kit be combined with qPCR for comprehensive CCL13 analysis?

Yes, this ELISA kit is ideal for quantifying CCL13 protein levels and can be effectively paired with qPCR to evaluate mRNA expression. Combining protein and transcriptional data provides a more complete understanding of macrophage inflammatory responses. We recommend collecting parallel samples for RNA and protein extraction to reduce variability between datasets.

Does the assay cover both basal and stimulated expression levels?

The kit offers a broad dynamic detection range suitable for measuring both baseline secretion and significant upregulation following inflammatory stimulation. Highly expressed samples can be diluted to fit within the standard curve range. Preliminary dilution testing helps ensure accurate absorbance readings and optimal data quality.

How long does the ELISA procedure require?

The ELISA protocol can typically be completed within 3-4 hours, including incubation and color development steps. When paired with qPCR validation, additional time for RNA extraction and amplification is required. The workflow remains practical for routine laboratory schedules.

Effective for combined protein and gene validation
We applied this kit alongside qPCR analysis in a macrophage activation study. The protein expression data correlated well with transcriptional changes, strengthening our experimental conclusions. The assay produced low background and consistent replicate values, supporting reliable data interpretation.
Wide dynamic range with clear differentiation
In our inflammation model, CCL13 expression varied substantially between treatment groups. This assay accurately captured both low and high expression levels. Even after sample dilution, the standard curve maintained strong linearity and reproducibility.
Helpful documentation and technical support
The protocol was detailed and easy to follow. During optimization, technical support provided useful advice regarding dilution strategy and curve fitting. The kit consistently generated stable and repeatable results across experiments.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.