In Vivo Lentivirus-based Macrophage Reprogramming Service for Inflammation

Targeting Macrophage for Inflammation

During inflammation, macrophages perform three main functions: antigen presentation, phagocytosis, and immune regulation. They regulate these processes by synthesizing various cytokines and growth factors to play functions. The wide range of physiologically active chemicals produced by macrophages and their products play a role in both the positive and negative effects of inflammation. Currently, targeting these cells with treatments could offer new approaches to managing inflammatory diseases.

Fig.1 Macrophage polarization state of activated macrophages.¹

Macrophage Polarization in Inflammation

Macrophage polarization is closely related to the development of inflammation and exhibits different characteristics as follows.

M1-like macrophage in inflammation	M2-like macrophage in resolution of inflammation
Marker with CD64 and CD60. Encourage Th1 polarization of CD4 cells. Phagocytic activity at a high level. Trigger acute inflammatory responses. The first line of protection against intracellular infections. Growth in inflammatory conditions with TLR and IFN signals.	Marker with CD64 and CD209. Highly endocytic and partially phagocytic. High expression levels of IL-8, MCP-1, IP-10, MIP-1β, and CCL5. M2a-like, M2b-like, and M2c-like are three subsets of m2-like macrophage based on different encountered stimuli. The regulator function reduces the production of cytokines that promote inflammation and increases the production of mediators that reduce inflammation.

M1-like macrophage in inflammation

M2-like macrophage in resolution of inflammation

Marker with CD64 and CD60.
Encourage Th1 polarization of CD4 cells.
Phagocytic activity at a high level.
Trigger acute inflammatory responses.
The first line of protection against intracellular infections.
Growth in inflammatory conditions with TLR and IFN signals.

Marker with CD64 and CD209.
Highly endocytic and partially phagocytic.
High expression levels of IL-8, MCP-1, IP-10, MIP-1β, and CCL5.
M2a-like, M2b-like, and M2c-like are three subsets of m2-like macrophage based on different encountered stimuli.
The regulator function reduces the production of cytokines that promote inflammation and increases the production of mediators that reduce inflammation.

Our In Vivo Lentivirus-based Macrophage Reprogramming Service for Inflammation

As a leader in the field, Creative Biolabs developed a cost-effective in vivo lentivirus-based macrophage reprogramming service for inflammation to aid in the macrophage application for inflammation disease treatment. In our service, we deliver the lentivirus vector expressing the desirable genes to engineer the macrophage in vivo. Importantly, the lentivirus-engineered macrophages not only strengthen their functions but also stably express therapeutic genes for a long time. During the entire process, we provide a full-around of services from lentivirus vector construction to outcomes presentation and customize the services to satisfy global customers' diverse demands.

In our service, we provide diverse gene targets to choose from for your projects, which include but are not limited to the following:

Fig.2 Target genes. (Creative Biolabs Original)

In addition, we also provide the lentivirus production service with strict control of quality for global customers. The following are the main quality control strategies in our service:

Virus production quality control: Comprehensive examination and quality control of virus-producing cell lines, viral seed strains, and culture conditions.
Virus purification quality control: The purity of virus products is an important aspect influencing product quality.
Virus quality evaluation: Examine the virus's specificity, integrity, activity, stability, and safety, as well as other indications, to assure the quality, safety, and efficacy of virus products.

Fig.3 Quality control. (Creative Biolabs Original)

Benefits for You

Here are the main advantages of our in vivo lentivirus-based macrophage reprogramming service for inflammation that is significantly useful for you.

Fig.4 Benefits of our in vivo lentivirus-based macrophage reprogramming service for inflammation. (Creative Biolabs Original)

Data Display

DATA 1-Targeting cTLR4

Background: The dysregulation of macrophages (MΦ) is increasingly being recognized as a risk factor for various inflammatory complications. However, the specific roles of pro-inflammatory (M1) macrophages and pro-healing (M2) macrophages have not yet been fully understood.

Method: Pro-inflammatory MΦ, MΦ-cTLR4 cells were engineered in this study by introducing the cTLR4 gene into the lentivirus vector. A tiny substance identified as the chemical inducer of dimerization (CID) medicine may be used to drive these cells to develop an MΦ phenotype comparable to MΦ.

Highlights: The results indicate that the pro-inflammatory MΦs designed in this study have the potential to effectively regulate inflammation. Moreover, by employing these MΦ-cTLR4 cells to regulate the host response, there is a potential to enhance angiogenesis, leading to improved healing results.

Fig.5 TNF, IL-6, and iNOS expression were increased in CID-treated MΦ-cTLR4 cells. (Eaton, et al., 2015) Fig.2 TNF, IL-6, and iNOS expression were increased in CID-treated MΦ-cTLR4 cells.²

Work with Creative Biolabs

Fig.6 Contact flowchart. (Creative Biolabs Original)

Here we provide an inquiry mode for your convenience. If you have an interest in our in vivo lentivirus-based macrophage reprogramming service for inflammation and would like more information, please don't hesitate to get in touch with us.

References

Atri, Chiraz, et al. "Role of human macrophage polarization in inflammation during infectious diseases." International journal of molecular sciences 19.6 (2018): 1801.
Eaton, K. V., et al. "Engineering macrophages to control the inflammatory response and angiogenesis." Experimental cell research 339.2 (2015): 300-309.