TNF-α Lentiviral Particle for Macrophage Engineering (MTS-0124-LX7)

Engineering Approach

Cytokine Gene Overexpression-Lentivirus

Species

Human

Target

TNF-α

Alternative Names

DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha

Full Name

Tumor necrosis factor

Introduction

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

UniprotID

GeneID

Applications

Positive regulator of M1 polarization of macrophage

Reference

Brempelis, Katherine J et al. "Genetically engineered macrophages persist in solid tumors and locally deliver therapeutic proteins to activate immune responses." Journal for immunotherapy of cancer vol. 8,2 (2020): e001356. doi:10.1136/jitc-2020-001356

Packaging System

Lentivirus

Titer

>1E8 TU/mL

Size

200uL; 1mL

Mycoplasma Testing

Negative

Sterility Testing

Negative

Shipping

Dry ice

Storage

Stored at -80°C for long term.

Handling Notes

Avoid multiple freeze/thaw cycles.

Restriction

RUO