Having a deep understanding of how the intestinal epithelium and the mucosal immune system interact is essential for promoting and preserving gut health. Based on our robust macrophage therapeutic platform, Creative Biolabs provides a specialized macrophage-enteroid coculture model development service that enables clients worldwide to delve deeper into the intricate communication between epithelial cells and macrophages. This innovative approach facilitates comprehensive studies on how these cell types work together to uphold barrier function and combat infections within the gut.
The intestinal epithelium is a dynamic and highly specialized barrier system essential for nutrient absorption and immune regulation. Enteroids—also known as intestinal organoids—are derived from intestinal stem cells and self-organize into 3D crypt-villus structures, recapitulating the architecture and function of the native epithelium. When cocultured with macrophages, these models provide a powerful platform to simulate the innate immune interface of the gut.
Traditional 2D epithelial monolayers and isolated macrophage cultures fail to capture the reciprocal, dynamic interactions that occur in vivo between these two key cell populations. By coculturing macrophages with enteroids, researchers can:
This model is particularly suited to simulating apical-basal transport, barrier disruption, inflammatory cascades, and tissue regeneration, providing a versatile tool for both mechanistic discovery and translational screening.
Recent studies utilizing macrophage–enteroid coculture models have shed light on:
At Creative Biolabs, we have pioneered the development of a macrophage-enteroid coculture model by 3D culturing human enteroid monolayers alongside mature human macrophages. Our approach involves inducing human intestinal stem cells to differentiate into enteroid monolayers, either sourced from patient. Additionally, we offer various macrophage cell lines, including patient-derived and monocyte-induced options, to enhance research flexibility. Noteworthy, our scientists are willing to customize your macrophage-enteroid coculture model development solutions to meet your needs. By leveraging these models, clients have the ability to explore immune responses in the gut microenvironment, paving the way for potential therapeutic strategies targeting inflammatory bowel diseases and gastrointestinal disorders.
We deliver end-to-end support for macrophage-enteroid coculture model development tailored to your research goals.
Process | Descriptions |
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Model Design & Customization |
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Coculture Establishment & Optimization |
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Assay Development & Analysis |
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Deliverables |
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During the course of research utilizing our macrophage-intestinal epithelial cell coculture model, researchers often require specific types of macrophages for further experiments. For this reason, we also offer a range of high-quality macrophage products designed to meet a variety of research needs.
Cat.No | Product Name | Product Type |
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MTS-0922-JF10 | Human Macrophages, Alveolar | Human Macrophages |
MTS-0922-JF99 | Human M0 Macrophages, 1.5 x 10^6 | Human M0 Macrophages |
MTS-0922-JF52 | C57/129 Mouse Macrophages, Bone Marrow | C57/129 Mouse Macrophages |
MTS-0922-JF7 | Human M2 Macrophages, Peripheral Blood, 10 x 10^6 | Human M2 Macrophages |
MTS-0922-JF34 | CD1 Mouse Macrophages | CD1 Mouse Macrophages |
MTS-0922-JF49 | C57BL/6 Mouse Macrophages (with LAB knockout), Bone Marrow | C57BL/6 Mouse Macrophages |
MTS-0922-JF43 | FVBN Mouse Macrophages, Bone Marrow | FVBN Mouse Macrophages |
MTS-0922-JF37 | BALBC Mouse Macrophages, Bone Marrow | BALBC Mouse Macrophages |
MTS-0922-JF33 | Balb/C Mouse Macrophages, Peripheral Blood,>5 x 10^6 | Balb/C Mouse Macrophages |
MTS-0922-JF11 | Cynomolgus Monkey Macrophages, Bone Marrow | Cynomolgus Monkey Macrophages |
Method: This study focused on investigating the interactions between intestinal epithelial cells and macrophages to understand how these cells collaborate in maintaining gut health. A novel research model was developed by co-culturing primary human macrophages with enteroid monolayers generated from intestinal stem cells, allowing for the study of interactions between immune cells and intestinal tissue. The model was utilized to assess barrier function, cytokine secretion, and protein expression in response to bacterial infection.
Result: The results suggest that the presence of macrophages significantly improved the barrier integrity and structure of enteroid monolayers, demonstrated by increases in cell height and transepithelial electrical resistance. The interaction between the epithelium and macrophages was established through observable morphological changes and cytokine secretion. Notably, the presence of intraepithelial macrophage projections, effective phagocytic activity, and the maintenance of enteroid barrier integrity showcased a coordinated response to infections caused by enterotoxigenic and enteropathogenic E. coli.
Fig.2 The establishment of macrophage-enteroid co-culture model.1,2
Q: What type of enteroid cells do you use? Are patient-derived organoids supported?
A: We offer two types of enteroid for coculture, including standard intestinal organoids derived from mice or humans and individualized intestinal organoids derived from specific patient samples. Customers can provide biopsy samples or iPSC-derived epithelial cells, and we will be responsible for isolating, expanding, and establishing the organoid culture system. In addition, we also support the use of commercially sourced standard gut organoid cell lines to shorten the development cycle.
Q: How are macrophages obtained? Is it a single source or can it be customized for research purposes?
A: We offer flexible macrophage sourcing options, including but not limited to:
Customers can choose between M0 unpolarized state or pre-induced M1/M2 polarized phenotype. We also support the induction of macrophage polarization during coculture for more realistic dynamic microenvironment simulation.
Q: How long is the service cycle usually? Does it support fast delivery or expedited processing?
A: The standard model development process includes model co-construction and validation phase, formal experimentation phase, data analysis and report delivery. If clients have clear time requirements, we can provide expedited processing channel.
Q: How does Creative Biolabs ensure the reproducibility and biological credibility of its models?
A: We follow a strict quality control system.
In addition, we have served more than dozens of international clients, including universities, pharmaceutical companies and research institutes, and have a proven track record of quality management and customer satisfaction.
References
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