Having a deep understanding of how the intestinal epithelium and the mucosal immune system interact is essential for promoting and preserving gut health. Based on our robust macrophage therapeutic platform, Creative Biolabs provides a specialized macrophage-enteroid coculture model development service that enables clients worldwide to delve deeper into the intricate communication between epithelial cells and macrophages. This innovative approach facilitates comprehensive studies on how these cell types work together to uphold barrier function and combat infections within the gut.

Macrophage-Enteroid Coculture Model Development Service at Creative Biolabs

Enteroids are advanced models that accurately replicate the complex cellular composition of intestinal tissue, offering valuable insights into developmental processes and diseases. These models are instrumental in high-throughput drug screening and customizing treatments for individuals.

At Creative Biolabs, we have pioneered the development of a macrophage-enteroid co-culture model by 3D culturing human enteroid monolayers alongside mature human macrophages. Our approach involves inducing human intestinal stem cells to differentiate into enteroid monolayers, either sourced from patient. Additionally, we offer various macrophage cell lines, including patient-derived and monocyte-induced options, to enhance research flexibility. Noteworthy, our scientists are willing to customize your macrophage-enteroid co-culture model development solutions to meet your needs. By leveraging these models, clients have the ability to explore immune responses in the gut microenvironment, paving the way for potential therapeutic strategies targeting inflammatory bowel diseases and gastrointestinal disorders.

Fig.1 Schematic diagram illustrating the process of creating a 2D monolayer of enteroids.¹

Benefits for You

• Specific approaches for various sources-derived cells.
• Excellent compatibility.
• Rapid turnaround.
• Stringent quality management.
• Customized solutions.

Published Data

Method: This study focused on investigating the interactions between intestinal epithelial cells and macrophages to understand how these cells collaborate in maintaining gut health. A novel research model was developed by co-culturing primary human macrophages with enteroid monolayers generated from intestinal stem cells, allowing for the study of interactions between immune cells and intestinal tissue. The model was utilized to assess barrier function, cytokine secretion, and protein expression in response to bacterial infection.

Result: The results suggest that the presence of macrophages significantly improved the barrier integrity and structure of enteroid monolayers, demonstrated by increases in cell height and transepithelial electrical resistance. The interaction between the epithelium and macrophages was established through observable morphological changes and cytokine secretion. Notably, the presence of intraepithelial macrophage projections, effective phagocytic activity, and the maintenance of enteroid barrier integrity showcased a coordinated response to infections caused by enterotoxigenic and enteropathogenic E. coli.

Fig.2 The establishment of macrophage-enteroid co-culture model.²

As a leader in cell biology and model construction, Creative Biolabs has accumulated experience in numerous projects and integrates it with promising macrophage co-culture model development. Thanks to that, our macrophage-enteroid co-culture model development service is able to effectively serve clients' meaningful projects. For additional information regarding this subject, please don't hesitate to reach out to us.