Macrophages-Epithelial Cell Coculture Model Development Service

Empowering Insights, One Coculture at a Time

Macrophage-epithelial cell coculture model provides researchers with a powerful tool to investigate the interactions between macrophages and epithelial cells comprehensively. Through carefully designed technical methods, highlighted advantages, and diverse applications, it facilitates a deeper understanding of cellular interactions, aiding drug development and disease treatment. Connect with Creative Biolabs for your macro-epithelial model needs!

Technical Methods of Macrophage-Epithelial Cell Coculture

By integrating a range of advanced technical methods, our macrophage-epithelial cell coculture model development service ensures the establishment of a sophisticated and physiologically relevant co-culture system for studying the intricate interactions between macrophages and epithelial cells. This comprehensive approach enables researchers to gain deeper insights into the complex biology of these cell types and their contributions to various physiological and pathological processes.

Technical Methods	Introduction
Cell Culture Optimization	Fine-tuning culture conditions, including media composition, supplements, and growth factors, to support the growth and viability of both cell types.
Cell Surface Modification	Employing surface modification techniques to enhance cell adhesion and interaction within the co-culture environment.
Microfluidic Systems Integration	Utilizing microfluidic systems to create dynamic co-culture environments that mimic physiological fluid flow and intercellular communication.
Immunofluorescence Imaging	Using immunofluorescence staining techniques to visualize and analyze spatial distribution and co-localization of cells within the co-culture model.
Gene Expression Profiling	Performing gene expression analysis to assess changes in gene expression patterns in response to co-culture, revealing molecular mechanisms.
Functional Assays	Conducting assays to evaluate biological activities such as cytokine secretion, phagocytic activity, and cell proliferation.
Live Cell Imaging	Employing live cell imaging techniques to dynamically monitor cellular behaviors and interactions in real-time within the co-culture system.

Applications

Studying interactions between macrophages and epithelial cells in inflammatory states elucidates the pathogenesis and treatment strategies of inflammatory diseases.
Investigating interactions between macrophages and epithelial cells in infectious diseases evaluates pathogen invasion mechanisms and immune responses.
Exploring interactions between macrophages and epithelial cells in tumor microenvironments elucidates tumor immune evasion mechanisms and immunotherapy strategies.

Advantages

Physiological environment simulation: The model accurately replicates the interactions between macrophages and epithelial cells under physiological conditions, offering a reliable platform for studying related biological processes.
Disease modeling and drug screening: The co-culture model can simulate interactions between macrophages and epithelial cells in various disease states, facilitating drug screening and evaluation.
Mechanism studies: In-depth investigation of the interaction mechanisms between macrophages and epithelial cells provides insights into the pathogenesis of related diseases and theoretical basis for therapeutic development.

Publications Sharing

Objective: Study host-pathogen interactions

Journal: Scientific reports

IF: 4.6

Research Findings: The author team has spearheaded the development and thorough characterization of an original primary human macrophage-enteroid co-culture model, providing a unique platform for investigating the complex interactions between epithelial cells and macrophages. Through compelling demonstrations of morphological shifts and cytokine release, the study illustrates robust communication dynamics between the epithelium and macrophages. These findings underscore the practicality and reproducibility of this innovative culture model, offering a promising avenue for delving into gut physiology and unraveling host responses to enteric pathogens.

Fig.1 Primary human macrophage-enteroid co-culture. (Gaelle, et al., 2017) Fig.1 Human enteroid monolayers and macrophages communicate in co-culture.¹

Tailored solutions at Creative Biolabs ensure accurate modeling of complex interactions between macrophages and epithelial cells, offering invaluable insights into various biological processes. Contact us to discuss how we can customize our macrophage-epithelial cell coculture model development services to meet your specific research needs.

Reference

Noel, Gaelle, et al. "A primary human macrophage-enteroid co-culture model to investigate mucosal gut physiology and host-pathogen interactions." Scientific reports 7.1 (2017): 45270.