Creative Biolabs offers a specialized model development service that explores the intricate crosstalk between macrophages and cancer cells, particularly in enabling cancer cells to acquire stem-like properties and develop chemoresistance. This service leverages our expertise in cell culture and model construction to bridge the gap in understanding the dynamics between these cells. By establishing a coculture model between cancer organoids and macrophages, we aim to unravel the underlying mechanisms of their interactions and provide invaluable insights for potential clinical applications.
Fig.1 Targeting the macrophage-CCL5-SP1-AREG loop decreases cancer stem-like characteristics and chemoresistance in pancreatic adenocarcinoma.1
The macrophage-organoid coculture system is an advanced in vitro model that bridges the gap between conventional 2D cultures and in vivo animal models. It enables a physiologically relevant recapitulation of tissue-specific immune microenvironments by combining two key elements:
This coculture model facilitates the study of:
Importantly, the platform supports both direct coculture (physical contact) and indirect coculture (e.g., transwell inserts), with optional integration of dynamic culture systems such as microfluidic chips for real-time monitoring of immune responses.
As a leading provider of customized cell-based models, Creative Biolabs is proud to offer a state-of-the-art macrophage-organoid coculture model development service. This service supports advanced preclinical research in immunology, oncology, infectious diseases, and regenerative medicine.
Creative Biolabs leverages expertise in organoid generation and myeloid cell biology to develop tailored macrophage-organoid coculture systems, customized to your research or drug screening needs. We offer an end-to-end development solution tailored to your experimental design and biological objectives.
Furthermore, our macrophage therapeutic platform includes a set of matured technologies, such as flow cytometry, western blot, etc. Backed up by these advanced technologies and our competent research team, Creative Biolabs is confident in delivering customized macrophage-organoid coculture models with good performance in a shorter turnaround.
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Parameter | Description |
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Physiological Accuracy | Mimics in vivo tissue architecture and macrophage activity |
High Customizability | Adaptable to different organ systems, macrophage phenotypes, and disease models |
Quantifiable Outcomes | Enables cytokine secretion profiling, barrier integrity assays, gene expression analysis |
Reduced Animal Use | Offers a humane and cost-effective alternative to in vivo testing |
Our macrophage-organoid coculture model provides a powerful tool for dissecting macrophage functionality and tissue-specific immune responses.
With these services, our goal is to advance macrophage research and allow researchers to focus more on scientific discovery without worrying about model construction and optimization. Next, we are pleased to introduce our newly launched macrophage products. Our macrophage product lineup includes macrophage cell lines and macrophage function assay kits.
Cat.No | Product Name | Product Type |
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MTS-1022-JF1 | B129 Mouse Bone Marrow Monocytes, 1 x 10^7 cells | Mouse Monocytes |
MTS-0922-JF99 | Human M0 Macrophages, 1.5 x 10^6 | Human M0 Macrophages |
MTS-0922-JF52 | C57/129 Mouse Macrophages, Bone Marrow | C57/129 Mouse Macrophages |
MTS-1022-JF6 | Human Cord Blood CD14+ Monocytes, Positive selected, 1 vial | Human Monocytes |
MTS-0922-JF34 | CD1 Mouse Macrophages | CD1 Mouse Macrophages |
MTS-1123-HM6 | Macrophage Colony Stimulating Factor (MCSF) ELISA Kit, Colorimetric | Detection Kit |
MTS-1123-HM15 | Macrophage Chemokine Ligand 19 (CCL19) ELISA Kit, qPCR | Detection Kit |
MTS-1123-HM17 | Macrophage Chemokine Ligand 4 (CCL4) ELISA Kit, Colorimetric | Detection Kit |
MTS-1123-HM49 | Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, Colorimetric | Detection Kit |
MTS-1123-HM42 | Macrophage Receptor with Collagenous Structure ELISA Kit, Colorimetric | Detection Kit |
We firmly believe that by providing high-quality products and services, we can help researchers achieve more fruitful results in the field of macrophages. If you have any questions about our macrophage-organoid coculture model service or related products, please feel free to contact us!
Background: | Gemcitabine resistance (GR) in pancreatic adenocarcinoma is influenced by interactions with macrophages in the tumor microenvironment. Investigating this relationship could lead to the discovery of innovative treatment strategies for addressing GR in pancreatic adenocarcinoma. |
Method: | Research is conducted to assess the impact of macrophage stimulation on gemcitabine resistance in pancreatic adenocarcinoma. Co-cultures involving patient-derived organoids (PDOs) from three PAAD cases and macrophages were implemented. Through analyzing data from various sources, including cytokine arrays, genomic databases, and single-cell sequencing data, researchers are able to uncover the signaling factors involved in the crosstalk between macrophages and pancreatic cancer cells, providing valuable insights into gemcitabine resistance mechanisms. |
Result: | This study emphasizes the importance of utilizing the PDO model for preclinical research and precision medicine. It reveals a macrophage-CCL5-Sp1-AREG feedback loop that enhances stemness in PCCs, contributing to GR through the activation of the CCR5/AKT/SP1/CD44 pathway. The synergistic effect of gemcitabine and mithramycin presents a promising therapeutic approach for PAAD treatment in various models, including cell lines, PDOs, and mouse studies. |
Fig.2 The CCL5/AREG loop enables communication between macrophages and PCCs.1
Q: What organoid types can you support? Does it include tumor organoids?
A: Our organoid platform supports organoids from multiple sources, including but not limited to:
Q: How long is the experimental cycle? Can you provide the experimental design and report?
A: The model development cycle depends on the organoid formation time, macrophage induction cycle, and co-culture duration. We provide the following support:
Q: Is it possible to construct individualized models?
A: Yes, we support personalized model construction using customer-provided samples, including:
Q: Are sample return shipments and long term storage services available?
A: Yes. We offer a variety of sample processing and follow-up services.
Customers have the flexibility to choose the sample processing method to support downstream experimental or publication needs.
References
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