Macrophage-Organoid Coculture Model Development Service
Overview Our Service Related Products Service Features Publications Scientific Resources Q & A

Creative Biolabs offers a specialized model development service that explores the intricate crosstalk between macrophages and cancer cells, particularly in enabling cancer cells to acquire stem-like properties and develop chemoresistance. This service leverages our expertise in cell culture and model construction to bridge the gap in understanding the dynamics between these cells. By establishing a coculture model between cancer organoids and macrophages, we aim to unravel the underlying mechanisms of their interactions and provide invaluable insights for potential clinical applications.

Fig.1 Targeting the macrophage-CCL5-SP1-AREG loop reduces cancer stem-like properties and GR in PAAD. (Jiang, et al., 2023)Fig.1 Targeting the macrophage-CCL5-SP1-AREG loop decreases cancer stem-like characteristics and chemoresistance in pancreatic adenocarcinoma.1

Overview of Macrophage-Organoid Coculture Model

The macrophage-organoid coculture system is an advanced in vitro model that bridges the gap between conventional 2D cultures and in vivo animal models. It enables a physiologically relevant recapitulation of tissue-specific immune microenvironments by combining two key elements:

  • Organoids: 3D mini-organs derived from tissue-specific stem cells or iPSCs, capable of self-organizing into structures that resemble native epithelial or organ tissues, such as the gut, liver, brain, or tumor tissues.
  • Macrophages: Innate immune cells derived from peripheral blood monocytes, iPSCs, or immortalized lines (e.g., THP-1), exhibiting high plasticity and functional diversity. Their phenotypes can be polarized to mimic disease-relevant immune states.

This coculture model facilitates the study of:

  • Immune-epithelial interactions under homeostatic and pathological conditions.
  • Macrophage polarization and trafficking in tissue-like structures.
  • Tissue damage, repair, and remodeling orchestrated by immune signals.
  • Pathogen-host interactions within a controlled and traceable microenvironment.

Importantly, the platform supports both direct coculture (physical contact) and indirect coculture (e.g., transwell inserts), with optional integration of dynamic culture systems such as microfluidic chips for real-time monitoring of immune responses.

As a leading provider of customized cell-based models, Creative Biolabs is proud to offer a state-of-the-art macrophage-organoid coculture model development service. This service supports advanced preclinical research in immunology, oncology, infectious diseases, and regenerative medicine.

Macrophage-Organoid Coculture Model Development Service at Creative Biolabs

Creative Biolabs leverages expertise in organoid generation and myeloid cell biology to develop tailored macrophage-organoid coculture systems, customized to your research or drug screening needs. We offer an end-to-end development solution tailored to your experimental design and biological objectives.

  • Model Design Consultation
    • Define organoid type (gut, brain, liver, etc.)
    • Choose macrophage source (primary, THP-1, iPSC-derived)
    • Select coculture format (direct, indirect, ALI, chip-based)
  • Cell Preparation
  • Coculture Establishment
    • Integration of immune cells into the organoid environment
    • Monitoring of cell viability, integration, and immune surveillance
  • Assay and Data Readouts
    • Cytokine and chemokine profiling (ELISA, multiplex assays)
    • Flow cytometry and immunofluorescence for polarization markers
    • RNA-seq, spatial transcriptomics, or single-cell analysis
    • Imaging of phagocytosis, migration, and tissue remodeling
  • Report Delivery
    • Full documentation of methods, QC, and reproducible protocols
    • High-resolution images and interactive datasets
    • Optional follow-up experimental consultation

Furthermore, our macrophage therapeutic platform includes a set of matured technologies, such as flow cytometry, western blot, etc. Backed up by these advanced technologies and our competent research team, Creative Biolabs is confident in delivering customized macrophage-organoid coculture models with good performance in a shorter turnaround.

Advanced Technologies at Creative Biolabs

  • ELISA
  • Sphere Formation Analysis
  • Flow Cytometry
  • Single-cell RNA-sequencing analysis
  • Western Blotting
  • Gene Expression Omnibus (GEO) analysis
  • Immunofluorescence
  • Cytokine Microarray Analysis

Key Advantages

Parameter Description
Physiological Accuracy Mimics in vivo tissue architecture and macrophage activity
High Customizability Adaptable to different organ systems, macrophage phenotypes, and disease models
Quantifiable Outcomes Enables cytokine secretion profiling, barrier integrity assays, gene expression analysis
Reduced Animal Use Offers a humane and cost-effective alternative to in vivo testing

Our macrophage-organoid coculture model provides a powerful tool for dissecting macrophage functionality and tissue-specific immune responses.

Related Products

With these services, our goal is to advance macrophage research and allow researchers to focus more on scientific discovery without worrying about model construction and optimization. Next, we are pleased to introduce our newly launched macrophage products. Our macrophage product lineup includes macrophage cell lines and macrophage function assay kits.

Cat.No Product Name Product Type
MTS-1022-JF1 B129 Mouse Bone Marrow Monocytes, 1 x 10^7 cells Mouse Monocytes
MTS-0922-JF99 Human M0 Macrophages, 1.5 x 10^6 Human M0 Macrophages
MTS-0922-JF52 C57/129 Mouse Macrophages, Bone Marrow C57/129 Mouse Macrophages
MTS-1022-JF6 Human Cord Blood CD14+ Monocytes, Positive selected, 1 vial Human Monocytes
MTS-0922-JF34 CD1 Mouse Macrophages CD1 Mouse Macrophages
MTS-1123-HM6 Macrophage Colony Stimulating Factor (MCSF) ELISA Kit, Colorimetric Detection Kit
MTS-1123-HM15 Macrophage Chemokine Ligand 19 (CCL19) ELISA Kit, qPCR Detection Kit
MTS-1123-HM17 Macrophage Chemokine Ligand 4 (CCL4) ELISA Kit, Colorimetric Detection Kit
MTS-1123-HM49 Macrophage Migration Inhibitory Factor (MIF) ELISA Kit, Colorimetric Detection Kit
MTS-1123-HM42 Macrophage Receptor with Collagenous Structure ELISA Kit, Colorimetric Detection Kit

We firmly believe that by providing high-quality products and services, we can help researchers achieve more fruitful results in the field of macrophages. If you have any questions about our macrophage-organoid coculture model service or related products, please feel free to contact us!

Service Features


20+ Years of Expertise
Unrivaled experience in cellular model development and immunoassays

Integrated Assay Platforms
From imaging to multi-omics, we provide full assay integration

Custom-built Solutions
Each project is tailored based on organ system, disease type, and client objectives

Global Support
Seamless communication and delivery for clients worldwide

Publications

Background: Gemcitabine resistance (GR) in pancreatic adenocarcinoma is influenced by interactions with macrophages in the tumor microenvironment. Investigating this relationship could lead to the discovery of innovative treatment strategies for addressing GR in pancreatic adenocarcinoma.
Method: Research is conducted to assess the impact of macrophage stimulation on gemcitabine resistance in pancreatic adenocarcinoma. Co-cultures involving patient-derived organoids (PDOs) from three PAAD cases and macrophages were implemented. Through analyzing data from various sources, including cytokine arrays, genomic databases, and single-cell sequencing data, researchers are able to uncover the signaling factors involved in the crosstalk between macrophages and pancreatic cancer cells, providing valuable insights into gemcitabine resistance mechanisms.
Result: This study emphasizes the importance of utilizing the PDO model for preclinical research and precision medicine. It reveals a macrophage-CCL5-Sp1-AREG feedback loop that enhances stemness in PCCs, contributing to GR through the activation of the CCR5/AKT/SP1/CD44 pathway. The synergistic effect of gemcitabine and mithramycin presents a promising therapeutic approach for PAAD treatment in various models, including cell lines, PDOs, and mouse studies.

Fig.2 The CCL5/AREG loop allows the communication between macrophages and PCCs. (Jiang, et al., 2023)Fig.2 The CCL5/AREG loop enables communication between macrophages and PCCs.1

Scientific Resources

Q & A

Q: What organoid types can you support? Does it include tumor organoids?

A: Our organoid platform supports organoids from multiple sources, including but not limited to:

  • Intestinal organoids (for studying intestinal inflammation, microbial-immune interactions)
  • Lung organoids (for respiratory disease modeling)
  • Liver organoids (for inflammation, toxicology, and metabolism studies)
  • Brain organoids (for neuroimmunology studies)
  • Tumor organoids (PDOs) (derived from patients or cell lines)

Q: How long is the experimental cycle? Can you provide the experimental design and report?

A: The model development cycle depends on the organoid formation time, macrophage induction cycle, and co-culture duration. We provide the following support:

  • Pre-technical communication and protocol development
  • Experimental design document (with flowchart and timeline)
  • Raw data records, pictures and graphs
  • Full experimental report with methodology, analyzed data and conclusion recommendations

Q: Is it possible to construct individualized models?

A: Yes, we support personalized model construction using customer-provided samples, including:

  • PBMC isolation and induction into autologous macrophages
  • Patient tumor tissue to create PDO-like organoids
  • Autoimmune cell-tumor co-culture to mimic individual immune response

Q: Are sample return shipments and long term storage services available?

A: Yes. We offer a variety of sample processing and follow-up services.

  • Co-culture cell clusters (organoid + macrophage) frozen and sent back
  • Sample return of molecular layer samples such as cell lysates, RNA, protein extracts, etc.
  • Paraffin embedded, frozen section return

Customers have the flexibility to choose the sample processing method to support downstream experimental or publication needs.

References

  1. Jiang, Shengwei, et al. "Macrophage-organoid co-culture model for identifying treatment strategies against macrophage-related gemcitabine resistance." Journal of Experimental & Clinical Cancer Research 42.1 (2023): 199. https://doi.org/10.1186/s13046-023-02756-4
  2. Distributed under Open Access license CC BY 4.0, without modification.
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