Tumor-associated Macrophage and Cancer Cell Coculture Model Development Service
Harmonizing Tumor Microenvironments: Explore Our Coculture Solutions for Deeper Cancer Insights!
By incorporating cutting-edge technologies, tumor-associated macrophage and cancer cell coculture model development services at Creative Biolabs provides a holistic approach to unraveling the complex interplay between tumor-associated macrophages (TAMs) and cancer cells in the tumor microenvironment.
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Key Technologies
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Details
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Cell Sourcing and Characterization
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Thorough selection and characterization of TAMs and cancer cells, considering their origin, phenotype, activation state, and functional properties.
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Culture Optimization
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Fine-tuning culture conditions such as media formulation, growth factors, and culture vessel geometry to create an environment conducive to the growth and interaction of TAMs and cancer cells.
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Biomimetic Coculture Systems
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Development of biomimetic coculture platforms that replicate the biochemical, mechanical, and spatial cues present in the tumor microenvironment, including 3D culture systems and organ-on-a-chip models.
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Functional Assays
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Implementation of functional assays to evaluate various aspects of TAM-cancer cell interactions, including cytokine secretion, immune cell infiltration, angiogenesis, and cancer cell invasion and metastasis.
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Imaging and Analysis
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Utilization of advanced imaging techniques such as confocal microscopy, live-cell imaging, and multiplex immunofluorescence to visualize and quantify TAM localization, phenotype, and behavior in the context of cancer cell interactions.
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Molecular Profiling
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Profiling of gene expression, protein expression, and signaling pathways in TAMs and cancer cells using techniques such as RNA sequencing, proteomics, and phosphoproteomics to elucidate the molecular mechanisms underlying their interaction.
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Drug Screening and Validation
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High-throughput screening of potential therapeutic compounds or biological agents targeting TAM-cancer cell interactions, followed by validation studies to assess efficacy and mechanism of action.
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Long-term Coculture Maintenance
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Establishment of protocols for long-term coculture maintenance, including regular media changes, cell passaging, and quality control assessments to ensure the stability and reproducibility of the coculture model over time.
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Applications
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Investigation of TAM-mediated immunosuppression, inflammation, and angiogenesis within the tumor microenvironment.
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Identification of novel therapeutic targets for disrupting TAM-cancer cell interactions and enhancing the efficacy of cancer immunotherapy and targeted therapy.
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Discovery of biomarkers associated with TAM infiltration and activation, providing prognostic indicators and potential targets for therapeutic intervention.
Advantages
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Relevance to Tumor Biology:
Accurate representation of the complex interactions between TAMs and cancer cells within the tumor microenvironment, providing insights into tumor progression, metastasis, and therapeutic resistance.
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Drug Screening and Validation:
A valuable platform for screening and validating potential therapeutic agents targeting TAM-cancer cell interactions, facilitating the development of novel cancer treatments.
Mechanistic Studies:
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Investigation of the molecular mechanisms underlying TAM-cancer cell interactions, elucidating key signaling pathways and cellular processes involved in tumor progression.
Personalized Medicine Applications:
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Potential applications in personalized medicine, allow for the testing of patient-derived samples to assess individualized responses to therapeutic interventions.
Publications Sharing
Journal: Scientific reports
IF: 37.3
Method: A laboratory co-culture experiment was conducted to examine how tumor-associated macrophages TAMs influence epithelial-mesenchymal transition (EMT), migration, and invasion in colorectal cancer (CRC). Additionally, various molecular techniques including ELISA, luciferase reporter assay, and chromatin immunoprecipitation (ChIP) were utilized to elucidate the underlying biological pathways. Furthermore, an animal model was employed to validate the impact of TAMs on metastasis mediated by mesenchymal circulating tumor cells (CTCs).
Research Findings: TAM and CRC cells cocoulture reveals a new cross-talk between immune cells and tumor cells in CRC microenvironment.
Fig.1 Schema for representing the experiment procedures.1
By leveraging cutting-edge techniques and comprehensive expertise, Creative Biolabs offers a tailored approach to unraveling the complexities of the tumor microenvironment. Contact us to unlock new insights into cancer biology and therapeutic strategies.
Reference
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Wei, Chen, et al. "Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis." Molecular cancer 18 (2019): 1-23.
