Immunity is a complex defense system in the body that protects against foreign invaders, such as pathogens and abnormal cells. Macrophages are specialized immune cells that engulf and destroy foreign substances, while T cells play a crucial role in coordinating the immune response. By working together, these two types of cells form a powerful defense network that can effectively target and eliminate threats to the body. Creative Biolabs' macrophage-T cell coculture model provides a valuable tool for studying the interactions between these immune cells in a controlled laboratory setting. This model allows researchers to investigate how macrophages and T cells communicate and collaborate to mount an effective immune response, leading to a better understanding of immune system function and potential therapeutic targets.

Fig.1 The interactions between TAMs and CD8⁺ T cells.¹

Macrophage-T Cell Coculture Model Development Service at Creative Biolabs

On the basis of the coculturing principle, our macrophage-T cell coculture model offers a unique platform to study the complex interactions between these two crucial components of the immune system. Our robust platform involves a number of cutting-edge technologies such as live-cell imaging and transcriptomic analysis to aid in gaining deeper insights into the dynamic interplay between macrophages and T cells. In addition, our team of experts is dedicated to optimizing experimental conditions and protocols, ensuring the reproducibility and reliability of our coculture models. We work closely with our customers to tailor our approach to your specific research goals, offering customized solutions and expert guidance every step of the way.

Representative Data

Cells: Bone marrow-derived macrophages & Splenic T cells

An important finding in cases of tissue damage, like stroke and burns, is the emergence of systemic immunosuppression marked by a decline in T cell populations and an increased susceptibility to infections. Scientists devised an in vitro macrophage-T cell coculture model to replicate the cellular interactions between innate and adaptive immune cells. The outcomes revealed that the demise of T cells following injury is instigated by the activation of inflammasomes within macrophages.

Fig.2 Analysis of bone marrow-derived macrophages–splenic T cells coculture through flow cytometric.²

Frequently Asked Questions

Q1: How to ensure the reliability and reproducibility of the established macrophage-T cell coculture model?

A1: Throughout the establishment of the macrophage-T cell coculture model, our adept research team will merge customers' specifications with operational viability, leveraging strengths to craft the development plan. By mitigating failure risks from the outset and meticulously documenting each facet of the project, we strive for utmost precision and replicability. Multiple iterations are conducted to validate the robustness and consistency of the coculture model.

Q2: What are the requirements for therapeutic compound screening with the macrophage-T cell coculture model development service?

A2: When utilizing the macrophage-T cell coculture model for compound screening, it is essential to maintain the purity, stability, and activity of the samples while ensuring an adequate quantity to fulfill experimental requirements and stability. It is advised to conduct concentration gradient testing beforehand to identify the optimal effective concentration range of the sample, preventing potential toxicity to cells due to excessive or insufficient concentration levels. Furthermore, taking into account the cell growth cycle and compound mechanisms, selecting a suitable time frame to assess the sample's impact is crucial. Regularly monitoring cell viability throughout the experimentation process is also vital.

For more details about our macrophage-T cell coculture model development service, please feel free to reach out to us.