The interaction between adipocytes and immune cells, particularly macrophages, leads to increased production of inflammatory and chemoattractant mediators, contributing to metabolic dysfunction locally and systemically. By utilizing co-culture models of adipocytes and macrophages, researchers can study how these interactions lead to inflammation and dysfunction in obese adipose tissue. With the support of our robust platform and seasoned team, Creative Biolabs brings out a macrophage-adipocyte coculture model development service to help in the development of therapeutic interventions targeting this cellular interaction.

Macrophage-Adipocyte Coculture Model Development Service at Creative Biolabs

Creative Biolabs' macrophage-adipocyte coculture model development service sheds light on advancing adipose tissue research, notably in personalized medicine strategies, therapeutic selection, and investigations into less common medical conditions.

Our service is uniquely designed to accommodate both primary cells and cell lines for the development of macrophage-adipocyte coculture models. We offer two main approaches: direct cell-cell culture and indirect culture using transwell inserts. Our team customizes the model development strategies according to the specific needs of our clients, ensuring optimal results. We utilize advanced technologies to thoroughly analyze and validate the models, including immunocytochemical markers, cell numbers, triglyceride accumulation, and protein secretion. By using our macrophage-adipocyte coculture models, clients can efficiently screen therapeutic compounds and strategies. Creative Biolabs is committed to providing high-quality results and detailed reports in a timely fashion for each client.

Fig.1 Illustration depicting alterations occurring in adipose tissue throughout obesity progression and fibrosis formation.¹

Cell Types for Macrophage-Adipocyte Coculture Model Development Service

Our macrophage-adipocyte coculture model development service can be performed not only on primary cells but similarly suitable for well-identified cell lines. Specific to cell lines, we deliver a variety of macrophage/monocyte cell lines and adipocyte cell lines that are widely applied for research to design for your projects.

• Primary Cells
• Cell Lines: THP-1; RAW264.7; U937 cell line; Murine 3T3-L1 preadipocyte cell line; etc.

Representative Data

Coculture Cells: RAW264.7 macrophage cell line & murine 3T3-L1 preadipocyte cell line

In this study, a macrophage-adipocyte coculture model is utilized to investigate the communication between adipose tissue and macrophages in the context of uremia. Findings indicate that adipocytes co-cultured with uremia-primed macrophages exhibit increased secretion of inflammatory cytokines compared to exposure to uremic sera alone. This suggests a synergistic relationship between uremia-primed macrophages and adipocytes.

Fig.2 Adipocytes show heightened production of inflammatory cytokines following exposure to uremic macrophages in a co-culture setting.²

Frequently Asked Question

Q: Which culture media would be most suitable for supporting macrophage growth and functions in experimental settings?

A: Research findings suggest that DME/F12 is the optimal culture medium for human macrophages, while IMDM is recommended for mouse macrophages. Further, variations in culture media can influence the balance between M1 and M2 macrophage phenotypes, emphasizing the importance of selecting the appropriate medium for specific experimental outcomes. At Creative Biolabs, we design tailored model development strategies, including the careful selection of culture media and differentiation agents, to meet the specific requirements of each project.

Whether you aim to delve into the complex interplay between macrophages and adipocytes or investigate potential treatments, our customized models are designed to propel your research endeavors forward. Contact us immediately for further information.